If workers must detect fluorescence from both RNA and DNA-bound Acridine Orange a longpass set such as 49012 is recommnded. 49011, 39002 may be used with LEDs with CWL 465-495nm; 49002 may be used with 455-485nm CWL LEDs.

49012 may be used with LEDs with CWL 465-495nm; 19002 may be used with 470-490nm CWL LEDs.

39009 may be used with LEDs with CWL 470-490nm; 49024 may be used with 430-450nm CWL LEDs.

Alexa Fluor 350™

49000 may be used with LEDs with CWL 350-365nm; 39000 may be used with 365-380nm CWL LEDs.

Alexa Fluor 405

49021 may be used with LEDs with CWL 400-410nm.

Alexa Fluor 488™

49011, 39002 may be used with LEDs with CWL 465-495nm; 49002 may be used with 455-485nm CWL LEDs.

Alexa Fluor 514™

49003, 39003 may be used with LEDs with CWL 490-505nm; 49023 may be use with 485-515nm CWL LEDs.

Alexa Fluor 532™

49014 may be used with LEDs with CWL520-540nm.

Alexa Fluor 546™

49004, 49305 may be used with LEDs with CWL 535-555nm; 39004 may be used with 535-550nm CWL LEDs.

Alexa Fluor 555™

49004, 49305 may be used with LEDs with CWL 535-555nm; 39004 may be used with 535-550nm CWL LEDs.

Alexa Fluor 568™

49031 may be used with LEDs with CWL from 560-580nm; 49008, 39010 may be used with 545-575nm LEDs.

Alexa Fluor 594™

49008, 39010 may be used with LEDs with CWL 545-575nm; 49306 may be used with 570-595nm CWL LEDs.

Alexa Fluor 610™

Alexa Fluor 633™

49015 may be used with LEDs with CWL 585-620nm.

Alexa Fluor 647™

49006 may be used with LEDs with CWL 595-640nm; 39007 may be used with 600-635nm CWL LEDs; 49009 may be used with 625-650nm CWL LEDs.

Alexa Fluor 680™

49022 may be used with LEDs with CWL 635-665nm.

Alexa Fluor 700™

49022 may be used with LEDs with CWL 635-665nm.

Alexa Fluor 750™

49007 may be used with LEDs with CWL 685-740nm.

Alexa Fluor 790™

49037 may be used with LEDs with CWL 725-755nm.

Allophycocyanin (APC)

49006 may be used with LEDs with CWL 595-640nm; 39007 may be used with 600-635nm CWL LEDs.

AmCyan

49001, 39001 may be used with LEDs with CWL 430-440nm.

AsRed2

49005, 49305 may be used with LEDs with CWL 535-555nm.

Atto 390

49028 may be used with LEDs with CWL385-405nm.

Atto 425

49013 may be used with LEDs with CWL 435-455nm; 39001 may be used with 430-440nm CWL LEDs.

Atto 465

49002 may be used with LEDs with CWL 455-485nm.

Atto 488

49011, 39002 may be used with LEDs with CWL 470-490nm; 49003 set may be used with 490-505nm LED.

Atto 550

49004, 49305 may be used with LEDs with CWL 535-555nm; 39004 may be used with 535-550nm CWL LEDs.

Atto 633

49015 may be used with LEDs with CWL 585-620nm.

Atto 647N

49006 may be used with LEDs with CWL 595-640nm; 39007 may be used with 600-635nm CWL LEDs; 49009 may be used with 625-650nm CWL LEDs.

Atto 680

49022 may be used with LEDs with CWL 635-665nm.

Auramine

JNO-19008 may be used with LEDs with CWL 435-470nm.

Auramine-rhodamine

19002 may be used with LEDs with CWL 470-490nm.

Azami Green

49002 may be used with LEDs with CWL 455-485nm; 49020 may be used with 475-485nm CWL LEDs; 39002 may be used with 470-490nm CWL LEDs.

BB515

49308 may be used with LEDs with CWL 475-495nm. 49303 may be used with 490-505nm CWL LEDs. 49303 is only recommended when multiplexing with BV480.

BCECF/pH 5.2

BCECF/pH 9.0

Biosearch Blue

49000 may be used with LEDs with CWL 350-365nm; 39000 may be used with 365-380nm CWL LEDs and 49028 may be used with 385-400nm CWL LEDs.

BODIPY FL/pH7.2

49011, 39002 may be used with LEDs with CWL 470-490nm; 49003 set may be used with 490-505nm LED.

Brilliant Violet™ 421

49027 may be used with LEDs with CWL 385-405nm.

Brilliant Violet™ 480

49032, 49302 and 49001 may be used with LEDs with CWL 430-440nm.

Brilliant Violet™ 510

Brilliant Violet™ 570

Brilliant Violet™ 605

Brilliant Violet™ 650

Brilliant Violet™ 711

Brilliant Violet™ 750

Brilliant Violet™ 786

Brilliant™ Ultraviolet 395

Brilliant™ Ultraviolet 496

Brilliant™ Ultraviolet 661

Brilliant™ Ultraviolet 737

Brilliant™ Ultraviolet 805

CAL Fluor® Gold 540

49023 may be use with LEDs with CWL 490-520nm; 49014 may be used with 520-540nm CWL LEDs.

CAL Fluor® Orange 560

49014 may be used with LEDs with CWL520-540nm; 49023 may be use with 490-520nm CWL LEDs.

CAL Fluor® Red 590

49031 may be used with LEDs with CWL from 560-580nm; 49005 may be used with 535-555nm CWL LEDs and 39004 may be used with 535-550nm CWL LEDs

CAL Fluor® Red 610

49306 may be used with LEDs with CWL 570-595nm; 49008, 39010 may be used with 545-575nm CWL LEDs.

CAL Fluor® Red 635

49015 may be used with LEDs with CWL 585-620nm.

Calcein

49011, 39002 may be used with LEDs with CWL 470-490nm; 49003 set may be used with 490-505nm LED.

Calcium Green™-1

49011, 39002 may be used with LEDs with CWL 470-490nm; 49003 set may be used with 490-505nm LED.

Calcofluor White

Calcofluor White is a commonly used stain to detect fungi and yeast and other microorganisms. It is a non-specific stain which preferentially binds to chitin and cellulose in cell walls and as such is also used to visualize plant cells. Calcofluor White has extremely broad excitation/emission spectra, and as a result almost any filter set with UV or violet/blue excitation wavelengths will generate fluorescence emission which may be detected across a very broad range. Some samples will fluoresce strongly in the green wavelengths, others in the blue, and others yellow-orange. Because of this, filter selection is subjective, partly depending on the experience of the microscopist. Our filter sets 19012, 19000, and 19011 may be used successfully depending on the sample and the observer’s preference. 19000 and 19012 may be used with LEDs with CWL 365-380nm; 19011 may be used with 395-410nm CWL LEDs.

Cerulean

49001, 39001 may be used with LEDs with CWL 430-440nm.

CFP

49001, 39001 may be used with LEDs with CWL 430-440nm.

Citrine

49003, 39003 may be used with LEDs with CWL 490-505nm.

Coumarin

49000 may be used with LEDs with CWL 350-365nm; 39000 may be used with 365-380nm CWL LEDs, 19011 may be used with 395-410nm CWL LEDs.

Cy2™

49011, 39002 may be used with LEDs with CWL 470-490nm; 49003 set may be used with 490-505nm LEDs.

Cy3.5™

49031 may be used with LEDs with CWL from 560-580nm; 49008, 39010 may be used with 545-575nm LEDs.

Cy3™

49004, 49005 may be used with LEDs with CWL 535-555nm; 39004 may be used with 535-550nm CWL LEDs.

Cy5.5™

49022 may be used with LEDs with CWL 635-665nm.

Cy5™

49006 may be used with LEDs with CWL 595-640nm; 39007 may be used with 600-635nm CWL LEDs; 49009 may be used with 625-650nm CWL LEDs.

Cy7™

49007 may be used with LEDs with CWL 685-740nm.

DAPI

49000 may be used with LEDs with CWL 350-365nm; 39000 may be used with 365-380nm CWL LEDs, 49028 may be used with 385-400nm CWL LEDs.

Di-8-ANEPPS non-ratiometric

39009 may be used with LEDs with CWL 470-490nm.

Di-8-ANEPPS, ratiometric

This dye and the related dye, Di-4-ANEPPS, are most effectively used as ratiometric indicators of membrane potential. Increases in membrane potential are indicated by a decrease in fluorescence intensity with approx. 440nm excitation and an increase in fluorescence intensity with approx. 530nm excitation. Use of this 71006 filter set requires an external filter wheel to house the 2 excitation filters and to facilitate rapid switching of filters for ratiometric measurements.

DiA

49024 may be used with LEDs with CWL 425-455nm; 39009, 19010 may be used with 470-490nm CWL LEDs.

DiD

49006 may be used with LEDs with CWL 595-640nm; 39007 may be used with 600-635nm CWL LEDs.

DiI

49004, 49305 may be used with LEDs with CWL 535-555nm; 39004 may be used with 535-550nm CWL LEDs.

DiO

49002 may be used with LEDs with CWL 455-485nm; 49020 may be used with 475-485nm CWL LEDs; 39002 may be used with 470-490nm CWL LEDs.

DiR

49007 may be used with LEDs with CWL 685-740nm.

Draq5

49006 may be used with LEDs with CWL 595-640nm; 39007 may be used with 600-635nm CWL LEDs.

DsRed

49004 may be used with LEDs with CWL 535-555nm; 39004 may be used with 535-550nm CWL LEDs.

DyLight 350

49000 may be used with LEDs with CWL 350-365nm; 39000 may be used with 365-380nm CWL LEDs.

DyLight 405

49021 may be used with LEDs with CWL 400-410nm; 19011 may be used with 395-410nm CWL LEDs.

DyLight 488

49011, 39002 may be used with LEDs with CWL 465-495nm; 49002 may be used with 455-485nm CWL LEDs.

DyLight 549

49004, 49305 may be used with LEDs with CWL 535-555nm; 39004 may be used with 535-550nm CWL LEDs.

DyLight 594

49008, 39010 may be used with LEDs with CWL 545-575nm; 49306 may be used with 570-595nm CWL LEDs.

DyLight 633

49015 may be used with LEDs with CWL 585-620nm.

DyLight 649

49006 may be used with LEDs with CWL 595-640nm; 39007 may be used with 600-635nm CWL LEDs; 49009 may be used with 625-650nm CWL LEDs.

DyLight 680

49022 may be used with LEDs with CWL 635-665nm.

DyLight 750

49007 may be used with LEDs with CWL 685-740nm.

DyLight 800

49037 may be used with LEDs with CWL 725-755nm.

EBFP2

49028 may be used with LEDs with CWL 385-400nm, 49021 may be used with 400-410nm CWL LEDs.

ECFP

49001, 39001 may be used with LEDs with CWL 430-440nm.

EGFP

49002 may be used with LEDs with CWL 455-485nm; 49020 may be used with 475-485nm CWL LEDs; 39002 may be used with 470-490nm CWL LEDs.

Emerald GFP

49002 may be used with LEDs with CWL 455-485nm; 49020 may be used with 475-485nm CWL LEDs; 39002 may be used with 470-490nm CWL LEDs.

Eosin

49023 may be used with LEDs with CWL 490-520nm.

Ethidium Bromide

49005 may be used with LEDs with CWL 535-555nm; 39009 may be used with 470-490nm CWL LEDs.

Ethidium homidimer-1/DNA

49005 may be used with LEDs with CWL 535-555nm; 39005 may be used with 535-550nm CWL LEDs.

EYFP/pH 7

49003, 39003 may be used with LEDs with CWL 490-505nm.

FAM

49011, 39002 may be used with LEDs with CWL 465-495nm; 49002 may be used with 455-485nm CWL LEDs.

FITC

49011, 39002 may be used with LEDs with CWL 465-495nm; 49002 may be used with 455-485nm CWL LEDs.

FlAsH-CCPFCC

49003, 39003 may be used with LEDs with CWL 490-505nm.

Fluo-3

49003 may be used with LEDs with CWL 490-505nm; 49011 may be used with 465-495nm CWL LEDs.

Fluo-4

49011, 39002 may be used with LEDs with CWL 465-495nm; 49002 may be used with 455-485nm CWL LEDs.

FluoroGold

This dye has mainly been used as a retrograde neuronal tracer, although it can also distinguish between DNA and RNA binding according to its fluorescence emission. With two excitation peaks at approx. 330nm and 390nm, and two emission peaks at approx. 450nm and 600nm, our DAPI longpass filter sets 49025 and 19012 are appropriate for detection of the fluorescence emission associated with the both peaks, which together appear “gold”. The 49025 set is also useful for distinguishing between FluoroGold bound to DNA or RNA.

FM™ 1-43

39009, 19010 may be used with LEDs with CWL 470-490nm.

FM™ 4-64

For imaging applications using a monochrome camera, this longpass filter set will not distinguish FM 4-64 from TRITC or similar orange/red fluorochromes. To be able to distinguish between FM 4-64 and TRITC or Texas Red-like fluorochromes, contact us for a custom filter set. 19010 may be used with LEDs with CWL 470-490nm; 19006 may be used with 545-575nm CWL LEDs.

Fura Red/Ca2+ – saturated

Fura Red/Ca2+ -free

Fura-2/Ca2+ – free

Fura-2/Ca2+ – saturated

FusionRed

49008, 39010 and 49017 may be used with LEDs with CWL 545-575nm.

GFP

EGFP spectra used here; similar to Emerald GFP, TagGFP, Azami Green, mWasabi, etc.49002 may be used with LEDs with CWL 455-485nm; 49020 may be used with 475-485nm CWL LEDs; 39002 may be used with 470-490nm CWL LEDs.

HEX,SE

49014 may be used with LEDs with CWL520-540nm; 49023 may be use with 485-515nm CWL LEDs.

Hoechst 33258

49000 may be used with LEDs with CWL 350-365nm; 39000 may be used with 365-380nm CWL LEDs.

Indo-1/Ca2+ -free

79006 may be used with LEDs with CWL 365nm.

Indo-1/Ca2+ -saturated

79006 may be used with LEDs with CWL 365nm.

Indocyanine Green

The spectral properties of ICG are strongly dependent on its concentration and the solute it is bound to, so care must be taken by workers to characterize their particular model system. This filter set 49030 is appropriate for most applications involving protein-bound ICG in various physiological media. ICG becomes concentrated in lipid environments, greatly shifting its spectral properties. 49030 may be used with LEDs with CWL 755-795nm.

JC-1 non-ratiometric

JC-1 exists as a monomer at low concentrations and/or low membrane potentials and displays fluorescence ex./em. maxima similar to that of YFP. At higher concentrations and/or higher membrane potentials, JC-1 forms aggregates with a red-shifted emission maxima of 590nm and a broadened excitation spectra. The 49012 longpass filter set will allow detection of JC-1 monomer and aggregate simultaneously. 49012 may be used with LEDs with CWL 465-495nm; 19002 may be used with 470-490nm CWL LEDs.

JC-1 ratiometric

JC-1 exists as a monomer at low concentrations and/or low membrane potentials and displays fluorescence ex./em. maxima similar to that of YFP. At higher concentrations and/or higher membrane potentials, JC-1 forms aggregates with a red-shifted emission maxima of 590nm and a broadened excitation spectra. Use of this 71019 filter set requires an external filter wheel to house the 2 emission filters to facilitate rapid switching of filters for ratiometric measurements.

JOE

49023 may be use with LEDs with CWL 485-515nm.

Killer Red

49008, 39010 may be used with LEDs with CWL 545-575nm; 49306 may be used with 570-595nm CWL LEDs.

Li-Cor IRDye® 680LT

49022 may be used with LEDs with CWL 635-665nm.

Li-Cor IRDye® 800CW

Lucifer Yellow

49026 may be used with LEDs with CWL 390-415nm; 39008 may be used with 405-430nm CWL LEDs.

LysoTracker Blue/MeOH

49028 may be used with LEDs with CWL 385-400nm; 49000 may be used with LEDs with CWL 350-365nm; 39000 may be used with 365-380nm CWL LEDs.

LysoTracker Green/pH 5.2

49002 may be used with LEDs with CWL 455-485nm; 49020 may be used with 475-485nm CWL LEDs; 39002 may be used with 470-490nm CWL LEDs.

LysoTracker Red/pH 5.2

49008 may be used with LEDs with CWL 545-575nm; 49305 may be used with 535-550nm CWL LEDs.

LysoTracker Yellow HCK-123

49023 may be used with LEDs with CWL 485-515nm; 39009 may be use with 470-490nm CWL LEDs.

mCherry

49008, 39010 may be used with LEDs with CWL 545-575nm; 49306 may be used with 570-595nm CWL LEDs.

mCitrine

49003, 39003 may be used with LEDs with CWL 490-505nm; 49023 may be use with 490-520nm CWL LEDs.

MitoTracker Deep Red 633/MeOH

49006 may be used with LEDs with CWL 595-640nm; 39007 may be used with 600-635nm CWL LEDs; 49009 may be used with 625-650nm CWL LEDs.

MitoTracker Green FM/MeOH

49002 may be used with LEDs with CWL 455-485nm; 49011 may be used with 465-495nm CWL LEDs; 39002 may be used with 470-490nm CWL LEDs.

MitoTracker Orange/MeOH

49004, 49305 may be used with LEDs with CWL 535-555nm; 39004 may be used with 535-550nm CWL LEDs.

MitoTracker Red/MeOH

49008, 39010 may be used with LEDs with CWL 545-575nm; 49306 may be used with 570-595nm CWL LEDs.

mKate2

49008, 39010 may be used with LEDs with CWL 545-575nm; 49306 may be used with 570-595nm CWL LEDs.

mKeima Red

49024 may be used with LEDs with CWL 430-450nm.

mKO

49014 may be used with LEDs with CWL 520-540nm; 49010 is not appropriate for use with LEDs because the bandwidth is too narrow.

mOrange2

49014 may be used with LEDs with CWL 520-540nm; 49010 is not appropriate for use with LEDs because the bandwidth is too narrow.

mPlum

49017, 19006 may be used with LEDs with CWL 545-575nm

mRFP1

49008, 39010 may be used with LEDs with CWL 545-575nm; 49306 may be used with 570-595nm CWL LEDs.

mTFP1

49013 may be used with LEDs with CWL 435-455nm.

mWasabi

49002 may be used with LEDs with CWL 455-485nm; 49020 may be used with 475-485nm CWL LEDs; 39002 may be used with 470-490nm CWL LEDs.

NBD X/MeOH

49011 may be used with LEDs with CWL 465-495nm; 19008 may be used with 435-465nm CWL LEDs.

Nile Blue

49006 may be used with LEDs with CWL 595-640nm; 39007 may be used with 600-635nm CWL LEDs.

NirFP

49019 may be used with LEDs with CWL 595-640nm.

Oregon Green™ 488

49011, 39002 may be used with LEDs with CWL 470-490nm; 49003 set may be used with 490-505nm LED.

Oregon Green™ 514

49003, 39003 may be used with LEDs with CWL 490-505nm. 49303 may be used with 490-505nm CWL LEDs.

Pacific Blue

49021 may be used with LEDs with CWL 400-410nm; 19011 may be used with 395-410nm CWL LEDs.

Propidium Iodide

49005 may be used with LEDs with CWL 535-555nm; 39005 may be used with 535-550nm CWL LEDs.

Pulsar™ 650

49024 may be used with LEDs with CWL 430-450nm; 39009, 19010 may be used with 470-490nm CWL LEDs.

Qdot 525

Although the excitation maxima of qdot nanocrystals is in the UV, a broad range of excitation wavelengths may be used.

Qdot 545

Although the excitation maxima of qdot nanocrystals is in the UV, a broad range of excitation wavelengths may be used.

Qdot 565

Although the excitation maxima of qdot nanocrystals is in the UV, a broad range of excitation wavelengths may be used.

Qdot 585

Although the excitation maxima of qdot nanocrystals is in the UV, a broad range of excitation wavelengths may be used.

Qdot 605

Although the excitation maxima of qdot nanocrystals is in the UV, a broad range of excitation wavelengths may be used.

Qdot 625

Although the excitation maxima of qdot nanocrystals is in the UV, a broad range of excitation wavelengths may be used.

Qdot 655

Although the excitation maxima of qdot nanocrystals is in the UV, a broad range of excitation wavelengths may be used.

Qdot 705

Although the excitation maxima of qdot nanocrystals is in the UV, a broad range of excitation wavelengths may be used.

Qdot 800

Although the excitation maxima of qdot nanocrystals is in the UV, a broad range of excitation wavelengths may be used.

Quasar® 570

49014 may be used with LEDs with CWL 520-540nm; 49304 may be used with 540-550nm CWL LEDs.

Quasar® 670

49006 may be used with LEDs with CWL 595-640nm; 39007 may be used with 600-635nm CWL LEDs; 49009 may be used with 625-650nm CWL LEDs.

Quasar® 705

49022 may be used with LEDs with CWL 635-670nm.

R-phycoerythrin

49014 may be used with LEDs with CWL 520-540nm; 19004 may be used with 535-545nm CWL LEDs; 49010 is not appropriate for use with LEDs because the bandwidth is too narrow.

ReAsH-CCPGCC

49008, 39010 may be used with LEDs with CWL 545-575nm; 49306 may be used with 570-595nm CWL LEDs.

Resorufin

49004, 49305 may be used with LEDs with CWL 535-555nm; 39004 may be used with 535-550nm CWL LEDs.

Rhod-2

49004, 49305 may be used with LEDs with CWL 535-555nm; 39004 may be used with 535-550nm CWL LEDs.

Rhodamine 123

49003, 39003 may be used with LEDs with CWL 490-505nm.

Rhodamine 6G

49023 may be use with LEDs with CWL 485-515nm.

Rhodamine Red™-X

49008, 39010 may be used with LEDs with CWL 545-575nm.

ROX

49008, 39010 may be used with LEDs with CWL 545-575nm.

49008, 39010 may be used with LEDs with CWL 545-575nm.

SYBR® Green I

49011, 39002 may be used with LEDs with CWL 465-495nm; 49002 may be used with 455-485nm CWL LEDs.

SYTO 9/DNA

49011, 39002 may be used with LEDs with CWL 465-495nm; 49002 may be used with 455-485nm CWL LEDs.

SYTO® 60

49006 may be used with LEDs with CWL 595-640nm; 39007 may be used with 600-635nm CWL LEDs; 49009 may be used with 625-650nm CWL LEDs.

T-Sapphire

TagBFP

49021 may be used with 400-410nm CWL LEDs, 49028 may be used with 385-400nm CWL LEDs.

TagRFP

49004, 49305 may be used with LEDs with CWL 535-555nm; 39004 may be used with 535-550nm CWL LEDs.

TAMRA

49004, 49305 may be used with LEDs with CWL 535-555nm; 39004 may be used with 535-550nm CWL LEDs.

tdTomato

49004, 49305 may be used with LEDs with CWL 535-555nm; 39004 may be used with 535-550nm CWL LEDs.

TET

49023 may be used with LEDs with CWL 485-515nm.

Tetracycline

Tetramethylrhodamine isothiocyanate

49004, 49305 may be used with LEDs with CWL 535-555nm; 39004 may be used with 535-550nm CWL LEDs.

Texas Red®

49008, 39010 may be used with LEDs with CWL 545-575nm; 49306 may be used with 570-595nm CWL LEDs.

Texas Red®-X

49008, 39010 may be used with LEDs with CWL 545-575nm; 49306 may be used with 570-595nm CWL LEDs.

TO-PRO™-3

49006 may be used with LEDs with CWL 595-640nm; 39007 may be used with 600-635nm CWL LEDs; 49009 may be used with 625-650nm CWL LEDs.

Topaz

49003, 39003 may be used with LEDs with CWL 490-505nm.

TRITC

49004, 49305 may be used with LEDs with CWL 535-555nm; 39004 may be used with 535-550nm CWL LEDs.

TurboFP650

49008, 49017 and 19006 may be used with LEDs with CWL 545-575nm.

wtGFP

19008 may be used with LEDs with CWL 435-470nm; 49002 may be used with 455-485nm CWL LEDs

XTop

X-rhod-1/Ca2+

49008, 39010 may be used with LEDs with CWL 545-575nm; 49306 may be used with 570-595nm CWL LEDs.

ZsGreen1

49002 may be used with LEDs with CWL 455-485nm; 49020 may be used with 475-485nm CWL LEDs; 39002 may be used with 470-490nm CWL LEDs.

ZsYellow

49023 may be use with LEDs with CWL 485-515nm; 49003 set may be used with 490-505nm LED.

– CKX53 –
Compact type Inverted Microscope

소형 도립 현미경

배양 세포 관찰

향상된 이미지 품질과 인체공학 설계로, Olympus CKX53은 라이브 셀 관찰, 세포 샘플링 및 처리, 이미지 캡처, 그리고 형광 관찰을 포함한 다양한 세포 배양 샘플에 뛰어난 성능과 효율적인 관찰 흐름을 제공합니다.

라이브 셀 관찰

통합 위상차( iPC : Integrated Phase Contrast ) 현미경

CKX53 iPC 시스템을 이용하여 대물렌즈 배율( 4x, 10x, 20x , 40x ) 변경시, 콘덴서 측의 위상차 링슬릿의 연동 변경이 필요 없게 되어 효율적인 관찰 작업이 가능하고, 또한 위상차 설정이 틀어짐에 대한 수시 조정이 필요없어 언제나 선명한 샘플 관찰이 가능합니다.

2X 배율, FN 22의 대물렌즈로 선명하고 넓은 시야

PLN2X 대물렌즈를 위한 링 슬릿, CKX3-SLPAS는 직경 11 ㎜ , 시야수 22 ㎜ 를 갖습니다.

2X 대물렌즈는 다른 대물렌즈 보다 확연히 높은 contrast를 제공하여, 투명한 샘플도 명확하게 식별할 수 있습니다. 예를 들어, 96-웰 마이크로 플레이트 관찰시, 넓은 시야로 인하여 스테이지를 움직이지 않고 웰의 모든 세포를 관찰 할 수 있습니다.

IVC (Inversion Contrast) 기술을 사용한 3D 셀 관찰

새로 개발된 IVC 기술로, 위상차보다 시야 심도는 좁아지며, 개체의 모양이나 투명도와 관계없이 삼차원 이미지를 선명하게 합니다. 또한, IVC 관찰은 후광 효과나, 방향성 있는 그림자를 배제하여, 개체의 선명한 관찰을 가능하게 합니다.
* 10X 대물렌즈 (PLCN10X, CACHN10XIPC)는 새로운 IVC 관찰에 사용할 수 있습니다.

Glass Heater for microscope

TPi-CKX53X ( Thermo Glass Plate )

Microscope: Olympus CKX53 series

Applicable stage: XY mechanical stage CKX3-MVR

Setting range: ambient ~ 60℃
Plate dimension: W190 x D138 ㎜
Heating area: W174 × D127 ㎜
Glass thickness: 0.5 ㎜

형광 관찰 (Fluorescence Microscopy)

다양한 형광 시약과 선명한 시야

100 W 수은 램프 (U-LH100HG), 130 W 고압 수은 램프 (U-HGLGPS), 그리고 타사(3^rd Party) LEDs*와 같은 여러 통합 광원을 이용하여 형광 이미지를 선명하게 관찰할 수 있습니다. 일반 연구용 형광현미경 IX3 및 BX3 에서 사용하는 미러 유닛을 동일하게 사용 할 수 있습니다.

3개의 형광미러 유닛을 장착할 수 있으나, Bright Field 관찰과 위상차 관찰에 영향을 줄 수 있으니 유닛 선택시 고려할 필요가 있습니다.

AcquCAM 23S2 Fluorescence camera DAPI, FITC, TRITC — Image taken by AcquCAM 23GR2 with LUCPlanFLN40x Ph2, 1x Adapter, CKX53

밝은 조건에서 높은 Contrast

“Umbra Shield”는 특히 CKX53을 사용한 형광 관찰을 위해 설계되었습니다. 차단막은 실내 광원을 효과적으로 차단하여 형광의 대비를 향상하여 밝은 실험실 조건에서도 선명한 형광 관찰이 가능합니다. 위상차를 사용하는 경우, Umbra 차단막을 들어 올려 표본에 빛을 통과시킬 수 있습니다.

형광관찰법 교육자료 with CKX53_by JHJIN다운로드

효율적인 세포 샘플링을 위한 사용자 중심 디자인

무균 조건에서의 효율적인 세포 관찰

CKX53 현미경은 UV 차단 코팅 덕분에 UV 살균 공정 중에 그대로 둘 수 있습니다. 이 시스템은 약 7kg (15.4lb)으로 이전 모델보다 가볍고 설치 공간이 더 작기 때문에 실험실 공간을 덜 차지합니다. 또한, 한 손으로 현미경을 움직일 수 있으며 관찰 경통의 목 부분을 이용하여 쉽게 운반 할 수 있습니다.

멸균 벤치 환경에서 간편한 세포 샘플링

CKX53의 접안렌즈와 광축/포커스 노브 사이의 거리가 짧으므로 작업자의 손의 위치를 자연스럽게 잡을 수 있어서 초점 및 셀 샘플링이 용이합니다.

다양한 세포 배양 용기를 사용할 수 있습니다.

CKX53의 공용 홀더로, 디쉬, 마이크로플레이트, 플라스크를 포함한 다양한 용기에서 배양된 세포를 확인하기 쉽습니다. 옵션 홀더가 부착되면, 최대 세 개의 35㎜ 배양 용기를 스테이지에 장착할 수 있습니다. 또한, 다양한 마이크로플레이트를 별도의 홀더 없이 다룰 수 있습니다.

다층 조직 플라스크(Multi-Layer Tissue Flask)를 위한 종합적인 관찰

CKX53의 폭과 탈착 가능한 콘덴서로 다층 조직 플라스크와 같은, 최대 190 ㎜ 높이의 배양 용기도 볼 수 있습니다. PLCN4X 대물렌즈의 우수한 초점 심도로 다층 조직 플라스크 내 바닥 두 개의 층의 세포를 빠르고 편하게 관찰할 수 있습니다.

다양한 용기를 사용하여 관찰 유연성 증대

홀더 암을 들어 올려서 수동으로 세포 배양 용기를 배치할 수 있습니다. 또한, 스테이지는 좌우로 최대 70 ㎜ 까지 확장할 수 있습니다.

Product_CKX53_07 — 홀더 암을 들어 올려서 수동으로 세포 배양 용기를 배치할 수 있습니다. 또한, 스테이지는 좌우로 최대 70 ㎜ 까지 확장할 수 있습니다.

데모장비 할인판매
OLYMPUS _ CKX53
( Evident )

Spec	Description
Size	1/1.8 inch
Resolution	H: 2048, V: 1536
Pixel size	H: 3.45 µm, V: 3.45 µm
Video formats& Frame rate	2048×1536 (3.1 MP) RGB64 @ 60 fps 2048×1536 (3.1 MP) RGB32 @ 60 fps 2048×1536 (3.1 MP) Y16 @ 60 fps 2048×1536 (3.1 MP) Y411 @ 60 fps 2048×1536 (3.1 MP) YUY2 @ 60 fps
Dynamic range	8 / 12 bit
Exposure time	20 ㎲ to 30s
Lens mount	C/Cs mount
Interface	USB 3.0
Power supply	4.5 to 5.5 VDC
Dimensions	H: 29 mm, W: 29 mm, L: 43 mm
Mass	65g

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