카테고리 보관물: MICROSCOPY

Fluorescence, Fluorochrome, MICROSCOPY

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Abberior Cage 500

Abberior Cage 532

Abberior Cage 590

Abberior Cage 635

Abberior Flip 565

Abberior Live 510

Abberior Live 515

Abberior Live 580

Abberior rsEGFP

Abberior rsEGFP2

Abberior Star 440SXP

Abberior Star 470SXP

Abberior Star 488

Abberior Star 512

Abberior Star 520SXP

Abberior Star 600

Abberior Star 635

Abberior Star 635p

Abberior Star Red

Acridine Orange + DNA

If workers must detect fluorescence from both RNA and DNA-bound Acridine Orange a longpass set such as 49012 is recommnded. 49011, 39002 may be used with LEDs with CWL 465-495nm; 49002 may be used with 455-485nm CWL LEDs.

Acridine Orange + DNA and RNA

49012 may be used with LEDs with CWL 465-495nm; 19002 may be used with 470-490nm CWL LEDs.

Acridine Orange + RNA

39009 may be used with LEDs with CWL 470-490nm; 49024 may be used with 430-450nm CWL LEDs.

Alexa Fluor 350™

49000 may be used with LEDs with CWL 350-365nm; 39000 may be used with 365-380nm CWL LEDs.

Alexa Fluor 405

49021 may be used with LEDs with CWL 400-410nm.

Alexa Fluor 488™

49011, 39002 may be used with LEDs with CWL 465-495nm; 49002 may be used with 455-485nm CWL LEDs.

Alexa Fluor 514™

49003, 39003 may be used with LEDs with CWL 490-505nm; 49023 may be use with 485-515nm CWL LEDs.

Alexa Fluor 532™

49014 may be used with LEDs with CWL520-540nm.

Alexa Fluor 546™

49004, 49305 may be used with LEDs with CWL 535-555nm; 39004 may be used with 535-550nm CWL LEDs.

Alexa Fluor 555™

49004, 49305 may be used with LEDs with CWL 535-555nm; 39004 may be used with 535-550nm CWL LEDs.

Alexa Fluor 568™

49031 may be used with LEDs with CWL from 560-580nm; 49008, 39010 may be used with 545-575nm LEDs.

Alexa Fluor 594™

49008, 39010 may be used with LEDs with CWL 545-575nm; 49306 may be used with 570-595nm CWL LEDs.

Alexa Fluor 610™

Alexa Fluor 633™

49015 may be used with LEDs with CWL 585-620nm.

Alexa Fluor 647™

49006 may be used with LEDs with CWL 595-640nm; 39007 may be used with 600-635nm CWL LEDs; 49009 may be used with 625-650nm CWL LEDs.

Alexa Fluor 680™

49022 may be used with LEDs with CWL 635-665nm.

Alexa Fluor 700™

49022 may be used with LEDs with CWL 635-665nm.

Alexa Fluor 750™

49007 may be used with LEDs with CWL 685-740nm.

Alexa Fluor 790™

49037 may be used with LEDs with CWL 725-755nm.

Allophycocyanin (APC)

49006 may be used with LEDs with CWL 595-640nm; 39007 may be used with 600-635nm CWL LEDs.

AmCyan

49001, 39001 may be used with LEDs with CWL 430-440nm.

AsRed2

49005, 49305 may be used with LEDs with CWL 535-555nm.

Atto 390

49028 may be used with LEDs with CWL385-405nm.

Atto 425

49013 may be used with LEDs with CWL 435-455nm; 39001 may be used with 430-440nm CWL LEDs.

Atto 465

49002 may be used with LEDs with CWL 455-485nm.

Atto 488

49011, 39002 may be used with LEDs with CWL 470-490nm; 49003 set may be used with 490-505nm LED.

Atto 550

49004, 49305 may be used with LEDs with CWL 535-555nm; 39004 may be used with 535-550nm CWL LEDs.

Atto 633

49015 may be used with LEDs with CWL 585-620nm.

Atto 647N

49006 may be used with LEDs with CWL 595-640nm; 39007 may be used with 600-635nm CWL LEDs; 49009 may be used with 625-650nm CWL LEDs.

Atto 680

49022 may be used with LEDs with CWL 635-665nm.

Auramine

JNO-19008 may be used with LEDs with CWL 435-470nm.

Auramine-rhodamine

19002 may be used with LEDs with CWL 470-490nm.

Azami Green

49002 may be used with LEDs with CWL 455-485nm; 49020 may be used with 475-485nm CWL LEDs; 39002 may be used with 470-490nm CWL LEDs.

BB515

49308 may be used with LEDs with CWL 475-495nm. 49303 may be used with 490-505nm CWL LEDs. 49303 is only recommended when multiplexing with BV480.

BCECF/pH 5.2

BCECF/pH 9.0

Biosearch Blue

49000 may be used with LEDs with CWL 350-365nm; 39000 may be used with 365-380nm CWL LEDs and 49028 may be used with 385-400nm CWL LEDs.

BODIPY FL/pH7.2

49011, 39002 may be used with LEDs with CWL 470-490nm; 49003 set may be used with 490-505nm LED.

Brilliant Violet™ 421

49027 may be used with LEDs with CWL 385-405nm.

Brilliant Violet™ 480

49032, 49302 and 49001 may be used with LEDs with CWL 430-440nm.

Brilliant Violet™ 510

Brilliant Violet™ 570

Brilliant Violet™ 605

Brilliant Violet™ 650

Brilliant Violet™ 711

Brilliant Violet™ 750

Brilliant Violet™ 786

Brilliant™ Ultraviolet 395

Brilliant™ Ultraviolet 496

Brilliant™ Ultraviolet 661

Brilliant™ Ultraviolet 737

Brilliant™ Ultraviolet 805

CAL Fluor® Gold 540

49023 may be use with LEDs with CWL 490-520nm; 49014 may be used with 520-540nm CWL LEDs.

CAL Fluor® Orange 560

49014 may be used with LEDs with CWL520-540nm; 49023 may be use with 490-520nm CWL LEDs.

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CAL Fluor® Red 590

49031 may be used with LEDs with CWL from 560-580nm; 49005 may be used with 535-555nm CWL LEDs and 39004 may be used with 535-550nm CWL LEDs

CAL Fluor® Red 610

49306 may be used with LEDs with CWL 570-595nm; 49008, 39010 may be used with 545-575nm CWL LEDs.

CAL Fluor® Red 635

49015 may be used with LEDs with CWL 585-620nm.

Calcein

49011, 39002 may be used with LEDs with CWL 470-490nm; 49003 set may be used with 490-505nm LED.

Calcium Green™-1

49011, 39002 may be used with LEDs with CWL 470-490nm; 49003 set may be used with 490-505nm LED.

Calcofluor White

Calcofluor White is a commonly used stain to detect fungi and yeast and other microorganisms. It is a non-specific stain which preferentially binds to chitin and cellulose in cell walls and as such is also used to visualize plant cells. Calcofluor White has extremely broad excitation/emission spectra, and as a result almost any filter set with UV or violet/blue excitation wavelengths will generate fluorescence emission which may be detected across a very broad range. Some samples will fluoresce strongly in the green wavelengths, others in the blue, and others yellow-orange. Because of this, filter selection is subjective, partly depending on the experience of the microscopist. Our filter sets 19012, 19000, and 19011 may be used successfully depending on the sample and the observer’s preference. 19000 and 19012 may be used with LEDs with CWL 365-380nm; 19011 may be used with 395-410nm CWL LEDs.

Cerulean

49001, 39001 may be used with LEDs with CWL 430-440nm.

CFP

49001, 39001 may be used with LEDs with CWL 430-440nm.

Citrine

49003, 39003 may be used with LEDs with CWL 490-505nm.

Coumarin

49000 may be used with LEDs with CWL 350-365nm; 39000 may be used with 365-380nm CWL LEDs, 19011 may be used with 395-410nm CWL LEDs.

Cy2™

49011, 39002 may be used with LEDs with CWL 470-490nm; 49003 set may be used with 490-505nm LEDs.

Cy3.5™

49031 may be used with LEDs with CWL from 560-580nm; 49008, 39010 may be used with 545-575nm LEDs.

Cy3™

49004, 49005 may be used with LEDs with CWL 535-555nm; 39004 may be used with 535-550nm CWL LEDs.

Cy5.5™

49022 may be used with LEDs with CWL 635-665nm.

Cy5™

49006 may be used with LEDs with CWL 595-640nm; 39007 may be used with 600-635nm CWL LEDs; 49009 may be used with 625-650nm CWL LEDs.

Cy7™

49007 may be used with LEDs with CWL 685-740nm.

DAPI

49000 may be used with LEDs with CWL 350-365nm; 39000 may be used with 365-380nm CWL LEDs, 49028 may be used with 385-400nm CWL LEDs.

Di-8-ANEPPS non-ratiometric

39009 may be used with LEDs with CWL 470-490nm.

Di-8-ANEPPS, ratiometric

This dye and the related dye, Di-4-ANEPPS, are most effectively used as ratiometric indicators of membrane potential. Increases in membrane potential are indicated by a decrease in fluorescence intensity with approx. 440nm excitation and an increase in fluorescence intensity with approx. 530nm excitation. Use of this 71006 filter set requires an external filter wheel to house the 2 excitation filters and to facilitate rapid switching of filters for ratiometric measurements.

DiA

49024 may be used with LEDs with CWL 425-455nm; 39009, 19010 may be used with 470-490nm CWL LEDs.

DiD

49006 may be used with LEDs with CWL 595-640nm; 39007 may be used with 600-635nm CWL LEDs.

DiI

49004, 49305 may be used with LEDs with CWL 535-555nm; 39004 may be used with 535-550nm CWL LEDs.

DiO

49002 may be used with LEDs with CWL 455-485nm; 49020 may be used with 475-485nm CWL LEDs; 39002 may be used with 470-490nm CWL LEDs.

DiR

49007 may be used with LEDs with CWL 685-740nm.

Draq5

49006 may be used with LEDs with CWL 595-640nm; 39007 may be used with 600-635nm CWL LEDs.

DsRed

49004 may be used with LEDs with CWL 535-555nm; 39004 may be used with 535-550nm CWL LEDs.

DyLight 350

49000 may be used with LEDs with CWL 350-365nm; 39000 may be used with 365-380nm CWL LEDs.

DyLight 405

49021 may be used with LEDs with CWL 400-410nm; 19011 may be used with 395-410nm CWL LEDs.

DyLight 488

49011, 39002 may be used with LEDs with CWL 465-495nm; 49002 may be used with 455-485nm CWL LEDs.

DyLight 549

49004, 49305 may be used with LEDs with CWL 535-555nm; 39004 may be used with 535-550nm CWL LEDs.

DyLight 594

49008, 39010 may be used with LEDs with CWL 545-575nm; 49306 may be used with 570-595nm CWL LEDs.

DyLight 633

49015 may be used with LEDs with CWL 585-620nm.

DyLight 649

49006 may be used with LEDs with CWL 595-640nm; 39007 may be used with 600-635nm CWL LEDs; 49009 may be used with 625-650nm CWL LEDs.

DyLight 680

49022 may be used with LEDs with CWL 635-665nm.

DyLight 750

49007 may be used with LEDs with CWL 685-740nm.

DyLight 800

49037 may be used with LEDs with CWL 725-755nm.

EBFP2

49028 may be used with LEDs with CWL 385-400nm, 49021 may be used with 400-410nm CWL LEDs.

ECFP

49001, 39001 may be used with LEDs with CWL 430-440nm.

EGFP

49002 may be used with LEDs with CWL 455-485nm; 49020 may be used with 475-485nm CWL LEDs; 39002 may be used with 470-490nm CWL LEDs.

Emerald GFP

49002 may be used with LEDs with CWL 455-485nm; 49020 may be used with 475-485nm CWL LEDs; 39002 may be used with 470-490nm CWL LEDs.

Eosin

49023 may be used with LEDs with CWL 490-520nm.

Ethidium Bromide

49005 may be used with LEDs with CWL 535-555nm; 39009 may be used with 470-490nm CWL LEDs.

Ethidium homidimer-1/DNA

49005 may be used with LEDs with CWL 535-555nm; 39005 may be used with 535-550nm CWL LEDs.

EYFP/pH 7

49003, 39003 may be used with LEDs with CWL 490-505nm.

FAM

49011, 39002 may be used with LEDs with CWL 465-495nm; 49002 may be used with 455-485nm CWL LEDs.

FITC

49011, 39002 may be used with LEDs with CWL 465-495nm; 49002 may be used with 455-485nm CWL LEDs.

FlAsH-CCPFCC

49003, 39003 may be used with LEDs with CWL 490-505nm.

Fluo-3

49003 may be used with LEDs with CWL 490-505nm; 49011 may be used with 465-495nm CWL LEDs.

Fluo-4

49011, 39002 may be used with LEDs with CWL 465-495nm; 49002 may be used with 455-485nm CWL LEDs.

FluoroGold

This dye has mainly been used as a retrograde neuronal tracer, although it can also distinguish between DNA and RNA binding according to its fluorescence emission. With two excitation peaks at approx. 330nm and 390nm, and two emission peaks at approx. 450nm and 600nm, our DAPI longpass filter sets 49025 and 19012 are appropriate for detection of the fluorescence emission associated with the both peaks, which together appear “gold”. The 49025 set is also useful for distinguishing between FluoroGold bound to DNA or RNA.

FM™ 1-43

39009, 19010 may be used with LEDs with CWL 470-490nm.

FM™ 4-64

For imaging applications using a monochrome camera, this longpass filter set will not distinguish FM 4-64 from TRITC or similar orange/red fluorochromes. To be able to distinguish between FM 4-64 and TRITC or Texas Red-like fluorochromes, contact us for a custom filter set. 19010 may be used with LEDs with CWL 470-490nm; 19006 may be used with 545-575nm CWL LEDs.

Fura Red/Ca2+ – saturated

Fura Red/Ca2+ -free

Fura-2/Ca2+ – free

Fura-2/Ca2+ – saturated

FusionRed

49008, 39010 and 49017 may be used with LEDs with CWL 545-575nm.

GFP

EGFP spectra used here; similar to Emerald GFP, TagGFP, Azami Green, mWasabi, etc.49002 may be used with LEDs with CWL 455-485nm; 49020 may be used with 475-485nm CWL LEDs; 39002 may be used with 470-490nm CWL LEDs.

HEX,SE

49014 may be used with LEDs with CWL520-540nm; 49023 may be use with 485-515nm CWL LEDs.

Hoechst 33258

49000 may be used with LEDs with CWL 350-365nm; 39000 may be used with 365-380nm CWL LEDs.

Indo-1/Ca2+ -free

79006 may be used with LEDs with CWL 365nm.

Indo-1/Ca2+ -saturated

79006 may be used with LEDs with CWL 365nm.

Indocyanine Green

The spectral properties of ICG are strongly dependent on its concentration and the solute it is bound to, so care must be taken by workers to characterize their particular model system. This filter set 49030 is appropriate for most applications involving protein-bound ICG in various physiological media. ICG becomes concentrated in lipid environments, greatly shifting its spectral properties. 49030 may be used with LEDs with CWL 755-795nm.

JC-1 non-ratiometric

JC-1 exists as a monomer at low concentrations and/or low membrane potentials and displays fluorescence ex./em. maxima similar to that of YFP. At higher concentrations and/or higher membrane potentials, JC-1 forms aggregates with a red-shifted emission maxima of 590nm and a broadened excitation spectra. The 49012 longpass filter set will allow detection of JC-1 monomer and aggregate simultaneously. 49012 may be used with LEDs with CWL 465-495nm; 19002 may be used with 470-490nm CWL LEDs.

JC-1 ratiometric

JC-1 exists as a monomer at low concentrations and/or low membrane potentials and displays fluorescence ex./em. maxima similar to that of YFP. At higher concentrations and/or higher membrane potentials, JC-1 forms aggregates with a red-shifted emission maxima of 590nm and a broadened excitation spectra. Use of this 71019 filter set requires an external filter wheel to house the 2 emission filters to facilitate rapid switching of filters for ratiometric measurements.

JOE

49023 may be use with LEDs with CWL 485-515nm.

Killer Red

49008, 39010 may be used with LEDs with CWL 545-575nm; 49306 may be used with 570-595nm CWL LEDs.

Li-Cor IRDye® 680LT

49022 may be used with LEDs with CWL 635-665nm.

Li-Cor IRDye® 800CW

Lucifer Yellow

49026 may be used with LEDs with CWL 390-415nm; 39008 may be used with 405-430nm CWL LEDs.

LysoTracker Blue/MeOH

49028 may be used with LEDs with CWL 385-400nm; 49000 may be used with LEDs with CWL 350-365nm; 39000 may be used with 365-380nm CWL LEDs.

LysoTracker Green/pH 5.2

49002 may be used with LEDs with CWL 455-485nm; 49020 may be used with 475-485nm CWL LEDs; 39002 may be used with 470-490nm CWL LEDs.

LysoTracker Red/pH 5.2

49008 may be used with LEDs with CWL 545-575nm; 49305 may be used with 535-550nm CWL LEDs.

LysoTracker Yellow HCK-123

49023 may be used with LEDs with CWL 485-515nm; 39009 may be use with 470-490nm CWL LEDs.

mCherry

49008, 39010 may be used with LEDs with CWL 545-575nm; 49306 may be used with 570-595nm CWL LEDs.

mCitrine

49003, 39003 may be used with LEDs with CWL 490-505nm; 49023 may be use with 490-520nm CWL LEDs.

MitoTracker Deep Red 633/MeOH

49006 may be used with LEDs with CWL 595-640nm; 39007 may be used with 600-635nm CWL LEDs; 49009 may be used with 625-650nm CWL LEDs.

MitoTracker Green FM/MeOH

49002 may be used with LEDs with CWL 455-485nm; 49011 may be used with 465-495nm CWL LEDs; 39002 may be used with 470-490nm CWL LEDs.

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MitoTracker Orange/MeOH

49004, 49305 may be used with LEDs with CWL 535-555nm; 39004 may be used with 535-550nm CWL LEDs.

MitoTracker Red/MeOH

49008, 39010 may be used with LEDs with CWL 545-575nm; 49306 may be used with 570-595nm CWL LEDs.

mKate2

49008, 39010 may be used with LEDs with CWL 545-575nm; 49306 may be used with 570-595nm CWL LEDs.

mKeima Red

49024 may be used with LEDs with CWL 430-450nm.

mKO

49014 may be used with LEDs with CWL 520-540nm; 49010 is not appropriate for use with LEDs because the bandwidth is too narrow.

mOrange2

49014 may be used with LEDs with CWL 520-540nm; 49010 is not appropriate for use with LEDs because the bandwidth is too narrow.

mPlum

49017, 19006 may be used with LEDs with CWL 545-575nm

mRFP1

49008, 39010 may be used with LEDs with CWL 545-575nm; 49306 may be used with 570-595nm CWL LEDs.

mTFP1

49013 may be used with LEDs with CWL 435-455nm.

mWasabi

49002 may be used with LEDs with CWL 455-485nm; 49020 may be used with 475-485nm CWL LEDs; 39002 may be used with 470-490nm CWL LEDs.

NBD X/MeOH

49011 may be used with LEDs with CWL 465-495nm; 19008 may be used with 435-465nm CWL LEDs.

Nile Blue

49006 may be used with LEDs with CWL 595-640nm; 39007 may be used with 600-635nm CWL LEDs.

NirFP

49019 may be used with LEDs with CWL 595-640nm.

Oregon Green™ 488

49011, 39002 may be used with LEDs with CWL 470-490nm; 49003 set may be used with 490-505nm LED.

Oregon Green™ 514

49003, 39003 may be used with LEDs with CWL 490-505nm. 49303 may be used with 490-505nm CWL LEDs.

Pacific Blue

49021 may be used with LEDs with CWL 400-410nm; 19011 may be used with 395-410nm CWL LEDs.

Propidium Iodide

49005 may be used with LEDs with CWL 535-555nm; 39005 may be used with 535-550nm CWL LEDs.

Pulsar™ 650

49024 may be used with LEDs with CWL 430-450nm; 39009, 19010 may be used with 470-490nm CWL LEDs.

Qdot 525

Although the excitation maxima of qdot nanocrystals is in the UV, a broad range of excitation wavelengths may be used.

Qdot 545

Although the excitation maxima of qdot nanocrystals is in the UV, a broad range of excitation wavelengths may be used.

Qdot 565

Although the excitation maxima of qdot nanocrystals is in the UV, a broad range of excitation wavelengths may be used.

Qdot 585

Although the excitation maxima of qdot nanocrystals is in the UV, a broad range of excitation wavelengths may be used.

Qdot 605

Although the excitation maxima of qdot nanocrystals is in the UV, a broad range of excitation wavelengths may be used.

Qdot 625

Although the excitation maxima of qdot nanocrystals is in the UV, a broad range of excitation wavelengths may be used.

Qdot 655

Although the excitation maxima of qdot nanocrystals is in the UV, a broad range of excitation wavelengths may be used.

Qdot 705

Although the excitation maxima of qdot nanocrystals is in the UV, a broad range of excitation wavelengths may be used.

Qdot 800

Although the excitation maxima of qdot nanocrystals is in the UV, a broad range of excitation wavelengths may be used.

Quasar® 570

49014 may be used with LEDs with CWL 520-540nm; 49304 may be used with 540-550nm CWL LEDs.

Quasar® 670

49006 may be used with LEDs with CWL 595-640nm; 39007 may be used with 600-635nm CWL LEDs; 49009 may be used with 625-650nm CWL LEDs.

Quasar® 705

49022 may be used with LEDs with CWL 635-670nm.

R-phycoerythrin

49014 may be used with LEDs with CWL 520-540nm; 19004 may be used with 535-545nm CWL LEDs; 49010 is not appropriate for use with LEDs because the bandwidth is too narrow.

ReAsH-CCPGCC

49008, 39010 may be used with LEDs with CWL 545-575nm; 49306 may be used with 570-595nm CWL LEDs.

Resorufin

49004, 49305 may be used with LEDs with CWL 535-555nm; 39004 may be used with 535-550nm CWL LEDs.

Rhod-2

49004, 49305 may be used with LEDs with CWL 535-555nm; 39004 may be used with 535-550nm CWL LEDs.

Rhodamine 123

49003, 39003 may be used with LEDs with CWL 490-505nm.

Rhodamine 6G

49023 may be use with LEDs with CWL 485-515nm.

Rhodamine Red™-X

49008, 39010 may be used with LEDs with CWL 545-575nm.

ROX

49008, 39010 may be used with LEDs with CWL 545-575nm.

SBFI/Na+ -free

SBFI/Na+ -saturated

SNARF pH 6.0

SNARF pH 9.0

Sulforhodamine 101

49008, 39010 may be used with LEDs with CWL 545-575nm.

SYBR® Green I

49011, 39002 may be used with LEDs with CWL 465-495nm; 49002 may be used with 455-485nm CWL LEDs.

SYTO 9/DNA

49011, 39002 may be used with LEDs with CWL 465-495nm; 49002 may be used with 455-485nm CWL LEDs.

SYTO® 60

49006 may be used with LEDs with CWL 595-640nm; 39007 may be used with 600-635nm CWL LEDs; 49009 may be used with 625-650nm CWL LEDs.

T-Sapphire

TagBFP

49021 may be used with 400-410nm CWL LEDs, 49028 may be used with 385-400nm CWL LEDs.

TagRFP

49004, 49305 may be used with LEDs with CWL 535-555nm; 39004 may be used with 535-550nm CWL LEDs.

TAMRA

49004, 49305 may be used with LEDs with CWL 535-555nm; 39004 may be used with 535-550nm CWL LEDs.

tdTomato

49004, 49305 may be used with LEDs with CWL 535-555nm; 39004 may be used with 535-550nm CWL LEDs.

TET

49023 may be used with LEDs with CWL 485-515nm.

Tetracycline

Tetramethylrhodamine isothiocyanate

49004, 49305 may be used with LEDs with CWL 535-555nm; 39004 may be used with 535-550nm CWL LEDs.

Texas Red®

49008, 39010 may be used with LEDs with CWL 545-575nm; 49306 may be used with 570-595nm CWL LEDs.

Texas Red®-X

49008, 39010 may be used with LEDs with CWL 545-575nm; 49306 may be used with 570-595nm CWL LEDs.

TO-PRO™-3

49006 may be used with LEDs with CWL 595-640nm; 39007 may be used with 600-635nm CWL LEDs; 49009 may be used with 625-650nm CWL LEDs.

Topaz

49003, 39003 may be used with LEDs with CWL 490-505nm.

TRITC

49004, 49305 may be used with LEDs with CWL 535-555nm; 39004 may be used with 535-550nm CWL LEDs.

TurboFP650

49008, 49017 and 19006 may be used with LEDs with CWL 545-575nm.

wtGFP

19008 may be used with LEDs with CWL 435-470nm; 49002 may be used with 455-485nm CWL LEDs

XTop

X-rhod-1/Ca2+

49008, 39010 may be used with LEDs with CWL 545-575nm; 49306 may be used with 570-595nm CWL LEDs.

ZsGreen1

49002 may be used with LEDs with CWL 455-485nm; 49020 may be used with 475-485nm CWL LEDs; 39002 may be used with 470-490nm CWL LEDs.

ZsYellow

49023 may be use with LEDs with CWL 485-515nm; 49003 set may be used with 490-505nm LED.

MICROSCOPY

LACTOBACILLUS INSPECTION

JNO-LACTO-CX43

유산균 관찰용 현미경

Remain Comfortable during Extended Usage for CX43 & CX33
Remain Comfortable during Extended Usage for CX43 & CX33
LACTOBACILLUS Inspection Microscope
LACTOBACILLUS Inspection Microscope with CX43

일반 광학 현미경의 관찰법(Bright Field)으로는 얇은 세포와 조직은 투명하여 배경과 구별이 잘 되지 않습니다. 이러한 문제를 해결하기 위해 샘플을 염색하여 관찰합니다만, 살아있는 샘플의 관찰이 필요한 경우에는 염색을 할 수 없습니다. (염색은 살아있는 샘플을 손상을 일으킵니다.)

이러한 살아있는 샘플의 관찰을 위하여 하기와 같은 다양한 관찰법들이 고안되었으며, OLYMPUS의 CX43은 소형 현미경이지만, 하기의 이미지와 같이 다양한 관찰법을 사용할 수 있어서 유산균 관찰과 같은 폭넓은 영역에서 사용되어 지고 있습니다.

Flexibility for Multiple Applications for CX43

– Brightfield
Flexibility for Multiple Applications for CX43

– Darkfield
Flexibility for Multiple Applications for CX43
Flexibility for Multiple Applications: for CX43
– Phase contrast

다양한 관찰법을 사용 가능한 유니버설 콘덴서
CX43은 유니버설 콘덴서를 탑재해 명시야 관찰, 암시야 관찰, 위상차 관찰, 형광 관찰, 간이 편광 관찰 등 다양한 관찰법에 사용가능합니다. 콘덴서는 2X에서 100X까지의 대물렌즈와 조합하여 관찰이 가능하고, 터렛 회전이나 탑 렌즈의 이동과 같은 번거로운 작업 없이 관찰 시간을 절약하고 조작의 실수를 줄일 수 있습니다.

Specimen Holders that Match Your Observation Style for CX43 & CX33
Specimen Holders that Match Your Observation Style for CX43 & CX33
Specimen Holders that Match Your Observation Style for CX43 & CX33
Specimen Holders that Match Your Observation Style for CX43 & CX33

관찰 스타일에 따른 크렌멜 제공
2개의 표본 동시에 끼워 비교하면서 관찰할 수 있는 크렌멜을 표준 장비해, 다양한 종류의 표본에 대응하고 있습니다. 옵션인 플레인 크렌멜은 표본을 홀더에 끼울 필요 없이 단지 표본을 시트 위에 놓기만 해도 쉽게 표본 이동이 가능합니다.

Contrast Enhancement with a Simple Aperture Stop Operation for CX43 & CX33
Contrast Enhancement with a Simple Aperture Stop Operation for CX43 & CX33
Contrast Enhancement with a Simple Aperture Stop Operation for CX43 & CX33

Aperture 조리개 조작으로 콘트라스트 강조
상기 이미지에서 우측 이미지와 같이 Aperture 조리개를 좁히는 것으로 contrast를 강조하는 효과를 얻을 수 있습니다.

Bright Field Image with CX43 & CX33
Bright Field Image with CX43
– Sample : kidney
Bright Field Image with CX43 & CX33
Bright Field Image with CX43
Sample : Tuberculosis bacteria
Bright Field Image with CX43 & CX33
Bright Field Image with CX43
Sample : Cervical cells
Darkfield Image with CX43 & CX33
Darkfield Image with CX43
Sample : Erythrocytes and leukocytes
Phase Contrast Image with CX43
Phase Contrast Image with CX43
– Sample : HeLa cells
Specification for CX43 ( OLYMPUS )
Specification for CX43 ( OLYMPUS )

CX43에 사용되는 카메라는 감도가 좋은 모델을 사용하시는 것이 좋습니다.
(하기와 같은 이유로 획득된 이미지가 밝지 못한 경우가 생기기 때문입니다.)
– 조명장치가 충분히 밝지 않음.
– 카메라로 가는 이미지 소스가 50%만 전달됨
– Dark Field와 위상차 관찰법은 간접광을 이용하거나, 링슬릿을 사용하기 때문에 Bright Field Microscopy에서 얻어지는 결과물에 비교하면 매우 어두운 관찰법입니다.

Microscope, MICROSCOPY, SIMULTANEOUS

BX51WI

ELECTROPHYSIOLOGY MICROSCOPE

AcquCAM Mono Camera for Physiology

CA3-pyramidal neuron_Alexa- Fluor-488 (This Image taken by AcquCAM 23S)
CA3-pyramidal neuron_Alexa- Fluor-488 (This Image taken by AcquCAM 23S)

Simultaneous observation
fluorescence observation image and IR-DIC image

Multi-Dual Port ( JNO-DPTS made by J.H.Jin )

JNOPTIC Multi-Dual Port System
IR-DIC 이미지와 형광 이미지의 실시간 동시 관찰
The optical design of this equipment was designed by J.H.JIN ( JNOPTIC Co., ltd )

AcquCAM 23S2 with Electro-Phyology

전기생리학 실험을 위한 패치 전용 카메라

with FV1200
AcquCAM 23S2
with BX51
with Nikon FN1
with Zeiss

전기생리학 전용 현미경 – OLYMPUS BX51WI / BX61WI

형광 이미징용 광원장치 – CoolLED pE-340fura

XY-MOVER & BRIDGE STAGE
(JNO-WI-3 made by JNOPTIC Co., ltd)

XY-MOVER

XY-Mover
with Zeiss Microscope
XY-MOVER made by JNOPTIC co.,ltd

전기생리학 전용 현미경 기능 개선

Accessories

Revolving nosepiece
Revolving nosepiece
Revolving nosepiece
DIC Prism & Analyzer
오염된 대물렌즈

전기생리학 – Best Image

CA3 pyramidal neuron with IR DIC Image
CA3 pyramidal neuron Alexa Fluor 488, IR-DIC (Simultaneous observations)
Figure courtesy by Dr. Sooyun Kim, Seoul National University

IR – DIC 촬영 조건

  • NIR Camera : AcquCAM 23S
  • Microscope : OLYMPUS BX51WI

IR-DIC 와 형광이미지의 동시 촬영

CA3 pyramidal neuron with IR DIC Image
CA3 pyramidal neuron
with IR-DIC
CA3 pyramidal neuron with Alexa Fluor 488
CA3 pyramidal neuron
with Alexa Fluor 488

Simultaneous observations ( IR – DIC & 형광 ) 촬영 조건

  • NIR Camera : AcquCAM 23S
  • Microscope : OLYMPUS BX51WI
  • Multi Dual Port : JNO-DPTS (개발자: J.H.JIN )

구형 OLYMPUS BX-WI의 이미지 업그레이드

NIKON 현미경의 이미지 개선 ( by J.H.JIN )

Before Ungrade
After Upgrade

업그레이드 전 업그레이드 후 이미지 비교

이미지의 상단을 클릭하시면 원본이미지를 보실 수 있습니다.

MICROSCOPY, SIMULTANEOUS

JNO-BF&FL

동시 관찰 검경법
– at the same time

Bright Field & Fluorescence Microscopy

명시야 검경법과 형광 검경법의 동시 관찰

본 발명은 현미경 관찰법 중에서 형광 이미징 관찰과 명시야 이미징 관찰을 동시에 수행할 수 있는 관찰 장치 및 관찰 방법을 제공합니다.

Simultaneous observation(Fluorescene & Bright Field microscopy

Explanation of patent technology –
Simultaneous observation of fluorescence microscopy and bright field microscopy
발명자: (주) 제이엔옵틱 진재환
Bright Field Image(Simultaneous observation)
Simultaneous observation of fluorescence microscopy and bright field microscopy
(동시관찰) 5만원 지폐의 표면의 명시야 관찰 (Image taken with AcquCAM 23GR2)
Fluorescene image(Simultaneous observation)
Simultaneous observation of fluorescence microscopy and bright field microscopy
(동시관찰) 5만원 지폐의 형광 관찰 (Image taken with AcquCAM 23GR2)
Bright Field Image(Simultaneous observation)
Simultaneous observation of fluorescence microscopy and bright field microscopy
(동시관찰) 5만원 지폐의 표면의 명시야 관찰 (Image taken with AcquCAM 23GR2)
Fluorescene image(Simultaneous observation)
Simultaneous observation of fluorescence microscopy and bright field microscopy
(동시관찰) 5만원 지폐의 형광 관찰 (Image taken with AcquCAM 23GR2)

Fluorescence, MICROSCOPY

Fluorescent Samples

댓글 3개

판매용 형광 샘플

Fluorescent Samples for Sale

이미지 상단부분을 클릭하시면 원본 이미지를 확인하실 수 있습니다.

판매용 형광 샘플 (U&B자극)
U & B 자극
판매용 형광 샘플 (U&G&R자극)
U & G & R 자극
판매용 형광 샘플 (B&G&R자극)
B & G & R 자극
판매용 형광 샘플 ((G&R자극)
G & R 자극
판매용 형광 샘플 (B자극)
B 자극
판매용 형광 샘플 (U자극)
U 자극
판매용 형광 샘플(U&B&G&R자극)
판매용 형광 샘플
-U & B & G & R자극 –

형광샘플 촬영 조건

  • 4ch 크로마 형광 필터
  • Camera: AcquCAM 23GR2
  • Objetives: UPlanApo40x
  • Camera Adapter: 1x
  • Light source: CoolLED pE-300ultra

education, MICROSCOPY

Polarization

편광 현미경

Polarization Microscopy

 

  • 편광현미경의 역사

19세기 중순 경 개발되어짐.

  • 편광현미경의 사용 용도

초기에는 암석과 광물의 연구에 주로 사용되어졌으나, 점차로 그 용도가 넓어져서 의약품, 공업제품 등 산업 전반에 걸쳐 이용분야가 확대 됨.

  • 편광현미경의 사용 목적

샘플의 광학적 성질을 조사하고,이를 통하여 샘플을 구성하는 물질이 무엇으로 이루어졌는지 동정 하기 위해 사용 됨.

  • 광학적 성질에 의한 샘플의 분류

광학적등방체

: 샘플에 빛이 통과 할때 어떠한 방향으로 빛이 진행하더라도 모든 방향에 대하여 동일한 광학적 영향을 준다.(복굴절 하지 않는다.)

예: 유리 등

광학적이방체

: 샘플에 빛이 통과 할때 빛이 진행하는 방향(각도)에 따라 다양한 복굴절을 한다.

일축성(isotropic body)

: 빛이 진행 할 때 복굴절하지 않는 광축을 하나만 가지고 있다.

예: 방해석, 석영 등

이축성(Anisotropic body)

: 빛이 진행 할 때 복굴절하지 않는 광축을 두개 가지고 있다.

 예: 운모, 장석, 각섬석, 휘석, 감람석 등

education, MICROSCOPY

Phase-contrast

위상차 현미경 관찰법

Phase contrast Microscopy

  • 위상차 관찰법 발명자 정보

– Frits Frederik Zernike(네덜란드 과학자)
– 위상차 현미경 발명으로 노벨물리학상(1953)을 수상.

  •  발명 배경

  생물현미경에서 사용하는 대부분의 샘플은 무색투명한 특성을 가지고 있기때문에,  배율확대 만을 목적으로 하는 일반 현미경의 관찰법(Bright Field)에서는 투명하고 윤곽이 흐릿하게 보이기 때문에 제대로 된 관찰에 어려움이 있습니다.

  이 문제를 해결하기 위하여 샘플을 염색하는 방법이 사용하고 있습니다만,  이 방법으로는 살아있는 샘플의 관찰은 할 수 없습니다. 염색 도중에 샘플이 죽어버리기 때문입니다.

  • 위상차 현미경의 (개요)

위상차 관찰법(Phase-Contrast)은 샘플을 통과하는 직진광(하단 좌측 이미지)과 이 직진광이 샘플에 통과하면서 발생하는 회절광(하단 우측 이미지) 사이의 위상차 현상을 이용하여 살아있는 세포의 구조와 미생물의 상태변화를 볼게 있게 해주는 관찰법입니다.

  • 위상차 현미경의 원리 설명

  샘플의 한 포인트(샘플 평면 중의 한 점)를 통과하는 빛은 두 개의 광학 경로를 가지도록 설계 되어 있으며, 이 두개의 경로를 통과한 빛은 한점에 다시 한 점에 모여 확대된 상을 만들게 되지만, 다른 경로를 지나왔기 때문에 발생한 위상차에 의하여 보강 또는 소멸 간섭을 하게 된다.

  하단의 좌측이미지는 두개의 광학경로 중에 직진광의 경로이며, 하단의 우측이미지는 직진광이 샘플에 닿을때 발생하는 회절광의 경로이다. 참고로 직진광이 샘플이 없는 포인트를 지나가게 되면 산란이 생기지않아 회절광은 발생하지 않는다.

  회절광은 직진광의 위상에 비교하여 대략 1/4λ 지연되어 결상한다. 직진광은 위상판에 의하여 1/4λ 또는 3/4λ지연되어 결상한다.

  직진광이 위상판에 의하여 1/4λ 지연되어 회절광과 동일한 위상을 갖게 되면 직진광과 회절광의 위상이 서로 보강간섭을 하여 진폭이 커지게 되면 배경에서는 직진광의 영향만 받기 때문에 샘플이 배경 보다 밝게 보인다. (Negative contrast)

  반대로  직진광이 위상판에 의하여 위상이 3/4λ 지연되면 직진광과 회절광은 소멸 간섭을 하게 되어 샘플은 직진광의 영향만 받는 배경보다 어둡게 보인다. (Positive contrast)

위상차관찰 전용 현미경 – CKX53

– 세포배양을 목적으로 하는 소형 위상차 현미경

MICROSCOPY

Hoffman Modulation Contrast

호프만 모듈레이션(HMC)

Hoffman Modulation Contrast Microscopy

  •  용도 및 특징

    • 기본적으로 위상차 현미경 관찰법, 미분간섭관찰법과 동일한 목적으로 사용되어 진다.  무색투명한 표본을 특수한 광학적 원리를 이용하여 가시화 한다.  이를 통하여 살아있는 세포의 구조와 미생물의 움직임을 관찰하는 것이 가능하다.
    • 비스듬한(경사진)각도의 조명법으로도 비슷한 느낌의 입체감을 얻을 수 있다. 
    • 초점심도가 얕아서 조금씩 초점을 바꿔주면 광학적인 절단상을 연속해서 얻을 수 있다.
    • 미분간섭 관찰과의 차이점은 플라스틱 샤레 등의 편광성이 있는 용기에서도 사용이 가능하다. 

  • 원리

    • 구성

      • HMC용 콘덴서
        • 콘덴서의 상부에 편광판을 회전가능하게 하여 둔다
        • 콘덴서의 전측 초첨위치에 슬릿을 배치한다.
        • 상기 슬릿은 대물렌즈와 매칭되도록 교환이 가능
      • HMC용 대물렌즈
        • 대물렌즈의 후측조점 위치에 HMC모듈레이를 설치한 전용 렌즈

 

MICROSCOPY

DIC microscopy (검경법)

미분 간섭 관찰 관찰법 ( DIC )

Differential Interference Contrast Microscopy(DIC)

  • Brightfield Image taken with
    olympus FV3100
  • Dic Image by JinOptic
    DIC Image taken with
    olympus FV3100
  • Brightfield Image taken with
    olympus FV3100 (clouseup)
  • DIC Image taken with
    olympus FV3100 (clouseup)
이미지를 클릭하시면 원본 이미지를 확인하실 수 있습니다.
  • 미분간섭관찰법 발명자

Georges (Jerzy) Nomarski (January 6, 1919 – 1997)

프랑스 귀화 물리학자 (폴란드 태생)

  • 발명 배경

이 관찰법은 샘플이 두꺼운 경우에 후광에 의한 광학 노이즈가 발현되는 위상차 현미경과 다르게 후광 효과가 발현되지 않으며, 샘플에 입체감을 부여하여 투명한 샘플에 대하여 보다 세밀한 관찰을 가능하게 합니다.

이 관찰법은 살아있는 생물학적 시료 및 염색을 하지 않은 조직의 관찰에 폭넓게 사용되고 있습니다.

  • DIC Microscopy 원리 설명

본 관찰법은 편광필터 2개(편광자, 검광자)와 DIC 프리즘 2개의 추가 유닛으로 구성되어 진다.

가장 바깥쪽에 위치하는 편광 필터 2개(편광자,검광자)는 Cross Nichole 상태로 설치 되어 있어야 하며, 이는 두개의 유닛 사이에 광학적 특성의 변화가 있는 빛만을 통과시키겠다는 목적으로 이해하면 된다.

 편광자를 통과한 빛이 처음 프리즘에 통과하면 빛은 2개의 경로를 가지게 된다. 이 두 빛은 광학진동 방향이 직교하며, 매우 미소한 거리 차를 가지고 진행한다.

샘플을 통과하고 다음 프리즘을 통과하면서 빛은 다시 동일한 경로를 갖는 빛으로 합성되며, 이때 다른 경로를 통과하였기 때문에 동일한 위상을 갖지 못하는 경우가 발생한다.

  동일한 위상일 경우에는 보강간섭 그렇지 않는 경우에는 소멸간섭이 발생한다.

DIC Microscopy 용어 설명(보강)

  • Phase Shift 
    1. 조정 방법: 1/4람다 필터 또는 DIC 프리즘에 있는 회전 레버을 이용하여 조정한다.
    2. 발생 효과: 보여 지는 샘플의 음영을 바꿀수 있다.
  • 쾰러 조명
    1. 최근에 사용되는 대부분의 관찰법은 쾰러 조명 세팅을 기본으로 하고 있음.
    2. 쾰러 조명을 기반으로 다양한 관찰법이 설계되어 있음.
Bright Field Microscopy와 DIC Microscopy의 이미지 비교
  • Brightfield Image
    투과조명
  • DIC Image
    투과조명
  • BF
    BrightField
    반사조명
  • differential interference contrast image
    DIC Image
    반사조명

별칭 : Normaski Interference Contrast Microscopy ( NIC )