– Filter set for Qdot625
– Chroma Filter Model No. : 39106
| Filters in this Set | Type |
|---|---|
| AT425/50x | EX |
| AT485DC | BS |
| AT625/30m | EM |
상기 필터를 구매하시기 전에, Excitation 영역에서 사용하시는 광원이 충분한 광량을 가지고 있는지 확인을 하셔야 하고, Emission영역이 가시광선 대역을 벗어나는 경우에는 검출장치(카메라)가 대응 가능한 영역인지 확인이 필요합니다.
현미경의 종류에 따라 Filter의 Size가 다르고, 같이 사용되어 지는 Cube의 종류가 다양하기 때문에, 구매시에는 반드시 판매 담당자와 충분한 확인을 하실 필요가 있습니다.
E-mail : jhjin@jnoptic.com
Mobile : + 82-2-3473-4188~9
AcquCAM Pro/G3
Down load Catalog _ AcquCAM PRO/G3
AcquCAM Pro/G3 USB 3.0 camera is the perfect solution for many bio, industrial, medical applications. Utilizing the very sensitive color CCD sensors, these cameras feature a specialization of fluorescence observations. With up to 30 fps and a trigger delay of less than 5 micro seconds.
LIVE PSEUDO COLOR
Ex. of applied PSEUDO COLOR for black live fluorescence image
Emphasis function on variety of images
Acquiring high sensitive images
Acquiring high sensitive images from general lighting source
[Specification]
| Spec | Description |
| Size | 1/3 inch |
| Resolution | H: 1280, V: 960 |
| Pixel size | H: 3.75 µm, V: 3.75 µm |
| Video formats & Frame rate | 1280×960 RGB32 @ 30, 20, 15, 7.5, 3.75 fps 1280×960 Y800 @ 30, 20, 15, 7.5, 3.75 fps 1280×960 Y16 @ 30, 20, 15, 7.5, 3.75 fps |
| Sensitivity | 0.05 lx |
| Exposure time | 1/10000 to 30 s |
| Lens mount | C/Cs mount |
| Interface | USB 3.0 |
| Power supply | 4.5 to 5.5 VDC |
| Dimensions | H: 29 mm, W: 29 mm, L: 43 mm |
| Mass | 65 g |
(Down load Catalog – AcquCAM 23S2)
AcquCAM 23S2 mono camera has a USB 3.0 interface and is the perfect solution for many industrial automation, quality assurance, security, surveillance and medical applications. The mono camera ships with the very sensitive 1/1.2 inch CMOS Pregius sensor. With up to 162 images per second, the AcuqCAM 23S2 is a low cost, yet highly versatile imaging solution. The camera includes a C to CS mount adapter, making it compatible to C and CS mount lenses.
| Spec | Description |
| Size | 1/1.2 inch |
| Resolution | H: 1920, V: 1200 |
| Pixel size | H: 5.86 µm, V: 5.86 µm |
| Video formats & Frame rate | 1920×1200 Y16 @ 80 fps 1920×1200 Y800 @ 162 fps |
| Sensitivity | 0.015 lx |
| Exposure time | 20㎲ to 30 s |
| Lens mount | C/Cs mount |
| Interface | USB 3.0 |
| Power supply | 4.5 to 5.5 VDC |
| Dimensions | H: 29 mm, W: 29 mm, L: 43 mm |
| Mass | 65 g |
Down load Catalog – ACQUCAM 3G
AcquCAM 3G color camera has a USB 3.0 interface and is the perfect solution for many industrial automation, quality assurance, security, surveillance and medical applications. The color camera ships with the very sensitive 1/1.8 inch CMOS Pregius sensor. With up to 60 images per second, the AcuqCAM 3G is a low cost, yet highly versatile imaging solution. The camera includes a C to CS mount adapter, making it compatible to C and CS mount lenses. Using the optional CS to M12 board lens adapter, the camera is
also compatible to M12 board lenses.
| Spec | Description |
| Size | 1/1.8 inch |
| Resolution | H: 2048, V: 1536 |
| Pixel size | H: 3.45 µm, V: 3.45 µm |
| Video formats& Frame rate | 2048×1536 (3.1 MP) RGB64 @ 60 fps 2048×1536 (3.1 MP) RGB32 @ 60 fps 2048×1536 (3.1 MP) Y16 @ 60 fps 2048×1536 (3.1 MP) Y411 @ 60 fps 2048×1536 (3.1 MP) YUY2 @ 60 fps |
| Dynamic range | 8 / 12 bit |
| Exposure time | 20 ㎲ to 30s |
| Lens mount | C/Cs mount |
| Interface | USB 3.0 |
| Power supply | 4.5 to 5.5 VDC |
| Dimensions | H: 29 mm, W: 29 mm, L: 43 mm |
| Mass | 65g |
Cy7™
| Excitation (㎚) | Emission (㎚) |
| 743 | 767 |
| Model No. | Excitation | Dichroic mirror | Emission | Remarks |
|---|---|---|---|---|
| JNO-49007 | ET710/75x | T760lpxr | ET810/90m | – |
| JNO-49037 | ET740/40x | T770lpxr | ET780lp | – |
Cyanines were and are still used in industry, and more recently in biotechnology (labeling, analysis). Cyanines have many uses as fluorescent dyes, particularly in biomedical imaging. Depending on the structure, they cover the spectrum from IR to UV.
Cyanine is the non-systematic name of a synthetic dye family belonging to polymethine group. The word cyanin is from the English word “cyan”, which conventionally means a shade of blue-green (close to “aqua”) and is derived from the Greek κυάνεος/κυανοῦς kyaneos/kyanous which means a somewhat different color: “dark blue”.
Cy7 is a near-IR fluor that is invisible to the naked eye (Excitation/emission maximum 750/776 ㎚). It is used in in vivo imaging applications, as well as the Cy7.5 dye.
Cyanines(Cy) replace advantageously conventional dyes such as Fluorescein(FITC) and rhodamines (TRITC, RRX), yielding brighter and more stable fluorescence.
시아닌은 여전히 업계에서, 그리고 최근에는 생명 공학 (라벨링, 분석)에서 사용되고 있습니다. 시아닌은 형광 염료로서, 특히 생체 의학 이미징에서 많은 용도로 사용되며 구조에 따라 IR에서 UV까지 스펙트럼을 커버합니다.
Cyanine은 polymethine 그룹에 속하는 합성 염료 계열의 비 계통적 이름입니다. cyanin이라는 단어는 일반적으로 청록색 ( “아쿠아”에 가까운)의 그늘을 의미하는 영어 단어 “cyan”에서 유래되었으며 다소 다른 색상 “진한 파란색 “을 의미하는 그리스어 κυάνεος / κυανοῦς kyaneos / kyanous에서 파생되었습니다.
Cy7은 육안으로는 보이지 않는 근적외선 형광체이며, Cy7.5 염료와 함께 생체 내 이미징 어플리케이션에 사용됩니다.
Cyanines (Cy)는 Fluorescein (FITC) 및 rhodamines (TRITC, RRX)와 같은 기존의 염료를 대체하여 보다 밝고 안정한 형광을 생성합니다.
이미지의 상단을 클릭하시면 원본 스펙트럼 차트를 보실 수 있습니다.
이미지 상단부분을 클릭하시면 원본 이미지를 확인하실 수 있습니다.
Compare filter sets by selecting the recommended/alternative set listed for each fluorochrome below.
하단의 Fluorochrome 이름을 클릭하시면
대응되는 형광필터의 조합을 확인 하실 수 있습니다.
Alexa Fluor 594 dye is a bright, red-fluorescent dye that can be excited using the 561 nm or 594 nm laser lines. For stable signal generation in imaging and flow cytometry, Alexa Fluor 594 dye is pH-insensitive over a wide molar range.
Cyan Fluorescent Protein (CFP) is a versatile biological marker for monitoring physiological processes, visualizing protein localization, and detecting transgenic expression in vivo. CFP can be excited by the 405 nm laser line and is optimally detected at 485 nm.
Cy5 dye is a bright, far-red-fluorescent dye that can be excited using the 633 or 647 nm laser lines, and visualized Cy5 filter sets. In addition to immunocytochemistry applications, Cy5 is also commonly used to label nucleic acids.
A variety of cyanine 5.5 (Cy5.5) dyes has been used to label biological molecules for fluorescence imaging and other fluorescence-based biochemical analysis. They are widely used for labeling peptides, proteins and oligos etc. Cy5.5 dyes are one type of the most common red fluorophores.
Cy7 is a near-IR fluor that is invisible to the naked eye (Excitation/emission maximum 750/776 nm). It is used in in vivo imaging applications, as well as the Cy7.5 dye.
DAPI (4′,6-diamidino-2-phenylindole) is a fluorescent stain that binds strongly to A-T rich regions in DNA. It is used extensively in fluorescence microscopy. As DAPI can pass through an intact cell membrane, it can be used to stain both live and fixed cells, though it passes through the membrane less efficiently in live cells and therefore the effectiveness of the stain is lower.
Fluorescein isothiocyanate (FITC) is a derivative of fluorescein used in wide-ranging applications including flow cytometry. FITC is the original fluoresce in molecule functionalized with an isothiocyanate reactive group (-N=C=S), replacing a hydrogen atom on the bottom ring of the structure. This derivative is reactive towards nucleophiles including amine and sulfhydryl groups on proteins.
The green fluorescent protein (GFP) is a protein composed of 238 amino acid residues (26.9 kDa) that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. Although many other marine organisms have similar green fluorescent proteins, GFP traditionally refers to the protein first isolated from the jellyfish Aequorea victoria. The GFP from A. victoria has a major excitation peak at a wavelength of 395 nm and a minor one at 475 nm. Its emission peak is at 509 nm, which is in the lower green portion of the visible spectrum.
tdTomato is a genetic fusion of two copies of the dTomato gene (2) which was specifi cally designed for low aggregation (1). Its tandem dimer structure plays an important role in the exceptional brightness of tdTomato (Table I). Its excitation and emission maxima occur at 554 nm and 581 nm, respectively (1). Because tdTomato forms an intramolecular dimer, it behaves like a monomer, and has been used successfully for N- and C- terminal fusions. It shows excellent photostability and its half-time (t0.5) for maturation is one hour at 37°C.
Tetramethylrhodamine (TRITC) is a bright orange-fluorescent dye with excitation ideally suited to the 532 nm laser line. It is commonly conjugated to antibodies and proteins for cellular imaging applications.
