카테고리 보관물: Fluorescence

JNO-Qdot625

– Filter set for Qdot625
– Chroma Filter Model No. : 39106

SpectraViewer – Qdot625
SpectraViewer - Qdot625
SpectraViewer – Qdot625
JNO-Qdot625
JNO-Qdot625
Chroma Model No. 39106
Filters in this SetType
AT425/50x EX
AT485DCBS
AT625/30mEM
JNO-Qdot625

상기 필터를 구매하시기 전에, Excitation 영역에서 사용하시는 광원이 충분한 광량을 가지고 있는지 확인을 하셔야 하고, Emission영역이 가시광선 대역을 벗어나는 경우에는 검출장치(카메라)가 대응 가능한 영역인지 확인이 필요합니다.


현미경의 종류에 따라 Filter의 Size가 다르고, 같이 사용되어 지는 Cube의 종류가 다양하기 때문에, 구매시에는 반드시 판매 담당자와 충분한 확인을 하실 필요가 있습니다.

E-mail : jhjin@jnoptic.com 
Mobile : + 82-2-3473-4188~9

AcquCAM Pro/G3

AcquCAM Pro/G3

Down load Catalog _ AcquCAM PRO/G3

AcquCAM Pro/G3

 AcquCAM Pro/G3 USB 3.0 camera is the perfect solution for many bio, industrial, medical applications. Utilizing the very sensitive color CCD sensors, these cameras feature a specialization of fluorescence observations. With up to 30 fps and a trigger delay of less than 5 micro seconds.

  • 1.2 Mega pixel CCD camera
  • Color, Progressive scan
  • Preview software provided (JNOPTIC Capture)
  • Software compatible with Windows XP/ Vista/ 7/ 8 operating systems

LIVE PSEUDO COLOR

Ex. of applied PSEUDO COLOR for black live fluorescence image

Emphasis function on variety of images

AcquCAM Pro G3_15
With emphasis function, BLACK BALANCE / AUTO LEVEL of ARM on variety images, you could acquire cleaner and sharper images.

Acquiring high sensitive images

These images were shot by AcquCAM Silver Package.

Acquiring high sensitive images from general lighting source

[Specification]

SpecDescription
Size1/3 inch
ResolutionH: 1280, V: 960
Pixel sizeH: 3.75 µm, V: 3.75 µm
Video formats   & Frame rate1280×960 RGB32 @ 30, 20, 15, 7.5, 3.75 fps   1280×960 Y800 @ 30, 20, 15, 7.5, 3.75 fps 1280×960 Y16 @ 30, 20, 15, 7.5, 3.75 fps
Sensitivity0.05 lx
Exposure time1/10000 to 30 s
Lens mountC/Cs mount
InterfaceUSB 3.0
Power supply4.5 to 5.5 VDC
DimensionsH: 29 mm, W: 29 mm, L: 43 mm
Mass65 g

AcquCAM 23S2

AcquCAM 23S2

(Down load Catalog – AcquCAM 23S2)

CA3 pyramidal neuron, IR-DIC & Alexa Fluo 488 at the same time
Figure courtesy by Dr. Sooyun Kim, Seoul National University
with Bx51WI, JNO-DPTS, AcquCAM 23S
JNO-DPTS
CA3 pyramidal neuron, IR-DIC & Alexa Fluo 488 at the same time
Figure courtesy by Dr. Sooyun Kim, Seoul National University
with Bx51WI, JNO-DPTS, AcquCAM 23S
  • USB 3.0 Monochrome Camera
  • 1/1.2 ” Pregius Sensor
  • 1920×1200 pixel
  • Trigger input and I/O
  • Cost-effective mono camera for fluorescence microscope (Best)
  • Cost-effective camera for Department of Physiology (Best)
  • 1/1.2 inch optimized sensor size for microscope field of view
  • Acquire images of optimum width combined with 0.63x adapter

AcquCAM 23S2 mono camera has a USB 3.0 interface and is the perfect solution for many industrial automation, quality assurance, security, surveillance and medical applications. The mono camera ships with the very sensitive 1/1.2 inch CMOS Pregius  sensor. With up to 162 images per second, the AcuqCAM 23S2 is a low cost, yet highly versatile imaging solution. The camera includes a C to CS mount adapter, making it compatible to C and CS mount lenses.

Images with AcquCAM 23S2
Sample : bovine pulmonary artery endothelial cell, BPAEC
Filter Set : JNO-U(B), B(B), G(B)
Camera : AcquCAM 23S2
C-mount adapter : 1x
Lens : UPlanAPO 40x
Above three images are merged and processed using ARM S/W
Sample : bovine pulmonary artery endothelial cell, BPAEC
Filter Set : JNO-U(B), B(B), G(B)
Camera : AcquCAM 23S2
C-mount adapter : 1x
Lens : UPlanAPO 40x
Above three images are merged and processed using ARM S/W

Specification

SpecDescription
  Size  1/1.2 inch
  Resolution  H: 1920, V: 1200
 Pixel size  H: 5.86 µm, V: 5.86 µm
 Video formats & Frame rate1920×1200  Y16    @    80 fps
1920×1200  Y800 @ 162 fps
  Sensitivity  0.015 lx
  Exposure time  20㎲ to 30 s
  Lens mount  C/Cs mount
  Interface  USB 3.0
  Power supply  4.5 to 5.5 VDC
  Dimensions  H: 29 mm, W: 29 mm, L: 43 mm
  Mass  65 g

AcquCAM 카메라 현미경 관찰법 대응표

AcquCAM 카메라 스펙 비교표

Setup Driver & Software Download

AcquCAM 3G

AcquCAM 3G

Down load Catalog – ACQUCAM 3G

  • USB 3.0 Color Camera
  • 1/1.8 ” Pregius Sensor
  • 2048 x 1536 pixel
  • Trigger input and I/O
  • Cost-effective color camera
  • High Sensitivity Sensor
  • Fast, seamless image transfer
  • Available of images with a very wide field of view
  • Low Noise Sensor

AcquCAM 3G color camera has a USB 3.0 interface and is the perfect solution for many industrial automation, quality assurance, security, surveillance and medical applications. The color camera ships with the very sensitive 1/1.8 inch CMOS Pregius  sensor. With up to 60 images per second, the AcuqCAM 3G is a low cost, yet highly versatile imaging solution. The camera includes a C to CS mount adapter, making it compatible to C and CS mount lenses. Using the optional CS to M12 board lens adapter, the camera is
also compatible to M12 board lenses.

Images with AcquCAM 3G

Sample : bovine pulmonary artery endothelial cell, BPAEC
Filter Set : JNO-U(B), B(B), G(B)
Camera : AcquCAM 3G
C-mount adapter : 1X
Lens : UPLNAPO40X
Above three images are merged and processed using ARM S/W

Specification

SpecDescription
Size1/1.8 inch
ResolutionH: 2048, V: 1536
Pixel sizeH: 3.45 µm, V: 3.45 µm
Video formats& Frame rate2048×1536 (3.1 MP) RGB64 @ 60 fps   2048×1536 (3.1 MP) RGB32 @ 60 fps
2048×1536 (3.1 MP) Y16 @ 60 fps 2048×1536 (3.1 MP) Y411 @ 60 fps
2048×1536 (3.1 MP) YUY2 @ 60 fps
Dynamic range8 / 12 bit
Exposure time20 ㎲ to 30s
Lens mountC/Cs mount
InterfaceUSB 3.0
Power supply4.5 to 5.5 VDC
DimensionsH: 29 mm, W: 29 mm, L: 43 mm
Mass65g

AcquCAM 카메라 현미경 관찰법 대응표

AcquCAM 카메라 스펙 비교표

Setup Driver & Software Download

Fluorochrome – Cy7™

Cy7™

Spectrum Chart

Cy7 Spectrum
Excitation (㎚)Emission (㎚)
743767

Recommended Filter set

  • 일반 형광 Lighting source
Model No.ExcitationDichroic mirrorEmissionRemarks
JNO-49007ET710/75xT760lpxrET810/90m
JNO-49037ET740/40xT770lpxrET780lp

Cy7 & JNO-49007 Specrtum Chart for CoollED pE4000
Cy7 & JNO-49037 Specrtum Chart for CoollED pE4000
  • Cy7을 관찰하기 위한 형광 광원 장치
    • T. 02-3473-4188로 연락 주시기 바랍니다.


Information

Cyanines were and are still used in industry, and more recently in biotechnology (labeling, analysis). Cyanines have many uses as fluorescent dyes, particularly in biomedical imaging. Depending on the structure, they cover the spectrum from IR to UV.

Cyanine is the non-systematic name of a synthetic dye family belonging to polymethine group. The word cyanin is from the English word “cyan”, which conventionally means a shade of blue-green (close to “aqua”) and is derived from the Greek κυάνεος/κυανοῦς kyaneos/kyanous which means a somewhat different color: “dark blue”.

Cy7 is a near-IR fluor that is invisible to the naked eye (Excitation/emission maximum 750/776 ㎚). It is used in in vivo imaging applications, as well as the Cy7.5 dye.

Cyanines(Cy) replace advantageously conventional dyes such as Fluorescein(FITC) and rhodamines (TRITC, RRX), yielding brighter and more stable fluorescence.

시아닌은 여전히 업계에서, 그리고 최근에는 생명 공학 (라벨링, 분석)에서 사용되고 있습니다. 시아닌은 형광 염료로서, 특히 생체 의학 이미징에서 많은 용도로 사용되며 구조에 따라 IR에서 UV까지 스펙트럼을 커버합니다.

Cyanine은 polymethine 그룹에 속하는 합성 염료 계열의 비 계통적 이름입니다. cyanin이라는 단어는 일반적으로 청록색 ( “아쿠아”에 가까운)의 그늘을 의미하는 영어 단어 “cyan”에서 유래되었으며 다소 다른 색상 “진한 파란색 “을 의미하는 그리스어 κυάνεος / κυανοῦς kyaneos / kyanous에서 파생되었습니다.

Cy7은 육안으로는 보이지 않는 근적외선 형광체이며, Cy7.5 염료와 함께 생체 내 이미징 어플리케이션에 사용됩니다.

Cyanines (Cy)는 Fluorescein (FITC) 및 rhodamines (TRITC, RRX)와 같은 기존의 염료를 대체하여 보다 밝고 안정한 형광을 생성합니다.

Fluorescent Samples

판매용 형광 샘플

Fluorescent Samples for Sale

이미지 상단부분을 클릭하시면 원본 이미지를 확인하실 수 있습니다.

형광샘플 촬영 조건

  • 4ch 크로마 형광 필터
  • Camera: AcquCAM 23GR2
  • Objetives: UPlanApo40x
  • Camera Adapter: 1x
  • Light source: CoolLED pE-300ultra

Fluorophores

Browse By Fluorochrome

Compare filter sets by selecting the recommended/alternative set listed for each fluorochrome below.

  • If you need more information, please click fluorochrome. You can see each fluorochrome information and recommended filter sets.
  • If you need other dye information, please contact us.
  • Tel : 02-3473-4188~9
  • E-mail : jhjin@jnoptic.com

하단의 Fluorochrome 이름을 클릭하시면
대응되는 형광필터의 조합을 확인 하실 수 있습니다.

AlexaFluor 594

Alexa Fluor 594 dye is a bright, red-fluorescent dye that can be excited using the 561 nm or 594 nm laser lines. For stable signal generation in imaging and flow cytometry, Alexa Fluor 594 dye is pH-insensitive over a wide molar range.

Spectrum-Chart_Alexa-Fluor-594
Spectrum-Chart_Alexa-Fluor-594

CFP

Cyan Fluorescent Protein (CFP) is a versatile biological marker for monitoring physiological processes, visualizing protein localization, and detecting transgenic expression in vivo. CFP can be excited by the 405 nm laser line and is optimally detected at 485 nm.

Spectrum-Chart_CFP
Spectrum-Chart_CFP

Cy5

Cy5 dye is a bright, far-red-fluorescent dye that can be excited using the 633 or 647 nm laser lines, and visualized Cy5 filter sets.  In addition to immunocytochemistry applications, Cy5 is also commonly used to label nucleic acids.

Spectrum-Chart_Cy5
Spectrum-Chart_Cy5

Cy5.5

A variety of cyanine 5.5 (Cy5.5) dyes has been used to label biological molecules for fluorescence imaging and other fluorescence-based biochemical analysis. They are widely used for labeling peptides, proteins and oligos etc. Cy5.5 dyes are one type of the most common red fluorophores.

Spectrum-Chart_Cy5.5
Spectrum-Chart_Cy5.5

Cy7

Cy7 is a near-IR fluor that is invisible to the naked eye (Excitation/emission maximum 750/776 nm). It is used in in vivo imaging applications, as well as the Cy7.5 dye.

Spectrum-Chart_Cy7
Spectrum-Chart_Cy7

DAPI

DAPI (4′,6-diamidino-2-phenylindole) is a fluorescent stain that binds strongly to A-T rich regions in DNA. It is used extensively in fluorescence microscopy. As DAPI can pass through an intact cell membrane, it can be used to stain both live and fixed cells, though it passes through the membrane less efficiently in live cells and therefore the effectiveness of the stain is lower.

Spectrum-Chart_DAPI
Spectrum-Chart_DAPI

FITC

Fluorescein isothiocyanate (FITC) is a derivative of fluorescein used in wide-ranging applications including flow cytometry. FITC is the original fluoresce in molecule functionalized with an isothiocyanate reactive group (-N=C=S), replacing a hydrogen atom on the bottom ring of the structure. This derivative is reactive towards nucleophiles including amine and sulfhydryl groups on proteins.

Spectrum-Chart_FITC
Spectrum-Chart_FITC

GFP

The green fluorescent protein (GFP) is a protein composed of 238 amino acid residues (26.9 kDa) that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. Although many other marine organisms have similar green fluorescent proteins, GFP traditionally refers to the protein first isolated from the jellyfish Aequorea victoria. The GFP from A. victoria has a major excitation peak at a wavelength of 395 nm and a minor one at 475 nm. Its emission peak is at 509 nm, which is in the lower green portion of the visible spectrum.

Spectrum-Chart_GFP
Spectrum-Chart_GFP

tdTomato

tdTomato is a genetic fusion of two copies of the dTomato gene (2) which was specifi cally designed for low aggregation (1). Its tandem dimer structure plays an important role in the exceptional brightness of tdTomato (Table I). Its excitation and emission maxima occur at 554 nm and 581 nm, respectively (1). Because tdTomato forms an intramolecular dimer, it behaves like a monomer, and has been used successfully for N- and C- terminal fusions. It shows excellent photostability and its half-time (t0.5) for maturation is one hour at 37°C.

TRITC

Tetramethylrhodamine (TRITC) is a bright orange-fluorescent dye with excitation ideally suited to the 532 nm laser line. It is commonly conjugated to antibodies and proteins for cellular imaging applications.

Spectrum-Chart_TRITC
Spectrum-Chart_TRITC

light source for Fluorescence

형광 관찰 조명장치의 종류

  • Mercury : 형광 현미경법에 가장 흔히 사용하는 광원으로, 자외선 및 가시광선 영역에서 아주 밝다.
Spectrum of mercury
  • Xenon : 가시광선에서 상대적으로 연속적인 스펙트럼을 가진다. 염료 및/또는 시료의 스펙트럼 특성을 정량적으로 분석하는 시스템에서는 제논 램프를 사용하는 것이 좋지만, 같은 와트수의 수은 램프만큼 밝지는 않다.
Spectrum of Xenon
  • LED :  약 10 ~ 40nm 범위의 상대적으로 좁은 스펙트럼 대역폭 내에서 빛을 방출한다는 고유의 특징이 있다. 일반적으로 정격 수명이 10,000시간 이상이고 50,000시간 이상 사용하는 경우도 많고, 전체 정격 수명에 걸쳐 출력 강도가 비교적 일정하게 유지된다.
Spectrum of LED

Laser : 레이저

  • 보통은 단색광의 좁은 스펙트럼 대역에서 극히 밝다. 하지만 레이저광의 간섭성으로 인해 샘플 영역에 균일한 조도로 비추기 어렵지만, Confocal 현미경에서는 갈바노 미러를 이용한 스캔 방식을 이용하여 이미지 획득시에 사용되어진다(LSCM).
    Spectrum of Laser

Metal-halide : 

  • 유효수명이 최대 2000시간으로, 일반적인 수은 램프의 200시간이나 제논 램프의 400시간과 비교하면 훨씬 길다.