카테고리 보관물: Fluorescence

Fluorescence, Fluorochrome, MICROSCOPY

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Abberior Cage 500

Abberior Cage 532

Abberior Cage 590

Abberior Cage 635

Abberior Flip 565

Abberior Live 510

Abberior Live 515

Abberior Live 580

Abberior rsEGFP

Abberior rsEGFP2

Abberior Star 440SXP

Abberior Star 470SXP

Abberior Star 488

Abberior Star 512

Abberior Star 520SXP

Abberior Star 600

Abberior Star 635

Abberior Star 635p

Abberior Star Red

Acridine Orange + DNA

If workers must detect fluorescence from both RNA and DNA-bound Acridine Orange a longpass set such as 49012 is recommnded. 49011, 39002 may be used with LEDs with CWL 465-495nm; 49002 may be used with 455-485nm CWL LEDs.

Acridine Orange + DNA and RNA

49012 may be used with LEDs with CWL 465-495nm; 19002 may be used with 470-490nm CWL LEDs.

Acridine Orange + RNA

39009 may be used with LEDs with CWL 470-490nm; 49024 may be used with 430-450nm CWL LEDs.

Alexa Fluor 350™

49000 may be used with LEDs with CWL 350-365nm; 39000 may be used with 365-380nm CWL LEDs.

Alexa Fluor 405

49021 may be used with LEDs with CWL 400-410nm.

Alexa Fluor 488™

49011, 39002 may be used with LEDs with CWL 465-495nm; 49002 may be used with 455-485nm CWL LEDs.

Alexa Fluor 514™

49003, 39003 may be used with LEDs with CWL 490-505nm; 49023 may be use with 485-515nm CWL LEDs.

Alexa Fluor 532™

49014 may be used with LEDs with CWL520-540nm.

Alexa Fluor 546™

49004, 49305 may be used with LEDs with CWL 535-555nm; 39004 may be used with 535-550nm CWL LEDs.

Alexa Fluor 555™

49004, 49305 may be used with LEDs with CWL 535-555nm; 39004 may be used with 535-550nm CWL LEDs.

Alexa Fluor 568™

49031 may be used with LEDs with CWL from 560-580nm; 49008, 39010 may be used with 545-575nm LEDs.

Alexa Fluor 594™

49008, 39010 may be used with LEDs with CWL 545-575nm; 49306 may be used with 570-595nm CWL LEDs.

Alexa Fluor 610™

Alexa Fluor 633™

49015 may be used with LEDs with CWL 585-620nm.

Alexa Fluor 647™

49006 may be used with LEDs with CWL 595-640nm; 39007 may be used with 600-635nm CWL LEDs; 49009 may be used with 625-650nm CWL LEDs.

Alexa Fluor 680™

49022 may be used with LEDs with CWL 635-665nm.

Alexa Fluor 700™

49022 may be used with LEDs with CWL 635-665nm.

Alexa Fluor 750™

49007 may be used with LEDs with CWL 685-740nm.

Alexa Fluor 790™

49037 may be used with LEDs with CWL 725-755nm.

Allophycocyanin (APC)

49006 may be used with LEDs with CWL 595-640nm; 39007 may be used with 600-635nm CWL LEDs.

AmCyan

49001, 39001 may be used with LEDs with CWL 430-440nm.

AsRed2

49005, 49305 may be used with LEDs with CWL 535-555nm.

Atto 390

49028 may be used with LEDs with CWL385-405nm.

Atto 425

49013 may be used with LEDs with CWL 435-455nm; 39001 may be used with 430-440nm CWL LEDs.

Atto 465

49002 may be used with LEDs with CWL 455-485nm.

Atto 488

49011, 39002 may be used with LEDs with CWL 470-490nm; 49003 set may be used with 490-505nm LED.

Atto 550

49004, 49305 may be used with LEDs with CWL 535-555nm; 39004 may be used with 535-550nm CWL LEDs.

Atto 633

49015 may be used with LEDs with CWL 585-620nm.

Atto 647N

49006 may be used with LEDs with CWL 595-640nm; 39007 may be used with 600-635nm CWL LEDs; 49009 may be used with 625-650nm CWL LEDs.

Atto 680

49022 may be used with LEDs with CWL 635-665nm.

Auramine

JNO-19008 may be used with LEDs with CWL 435-470nm.

Auramine-rhodamine

19002 may be used with LEDs with CWL 470-490nm.

Azami Green

49002 may be used with LEDs with CWL 455-485nm; 49020 may be used with 475-485nm CWL LEDs; 39002 may be used with 470-490nm CWL LEDs.

BB515

49308 may be used with LEDs with CWL 475-495nm. 49303 may be used with 490-505nm CWL LEDs. 49303 is only recommended when multiplexing with BV480.

BCECF/pH 5.2

BCECF/pH 9.0

Biosearch Blue

49000 may be used with LEDs with CWL 350-365nm; 39000 may be used with 365-380nm CWL LEDs and 49028 may be used with 385-400nm CWL LEDs.

BODIPY FL/pH7.2

49011, 39002 may be used with LEDs with CWL 470-490nm; 49003 set may be used with 490-505nm LED.

Brilliant Violet™ 421

49027 may be used with LEDs with CWL 385-405nm.

Brilliant Violet™ 480

49032, 49302 and 49001 may be used with LEDs with CWL 430-440nm.

Brilliant Violet™ 510

Brilliant Violet™ 570

Brilliant Violet™ 605

Brilliant Violet™ 650

Brilliant Violet™ 711

Brilliant Violet™ 750

Brilliant Violet™ 786

Brilliant™ Ultraviolet 395

Brilliant™ Ultraviolet 496

Brilliant™ Ultraviolet 661

Brilliant™ Ultraviolet 737

Brilliant™ Ultraviolet 805

CAL Fluor® Gold 540

49023 may be use with LEDs with CWL 490-520nm; 49014 may be used with 520-540nm CWL LEDs.

CAL Fluor® Orange 560

49014 may be used with LEDs with CWL520-540nm; 49023 may be use with 490-520nm CWL LEDs.

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CAL Fluor® Red 590

49031 may be used with LEDs with CWL from 560-580nm; 49005 may be used with 535-555nm CWL LEDs and 39004 may be used with 535-550nm CWL LEDs

CAL Fluor® Red 610

49306 may be used with LEDs with CWL 570-595nm; 49008, 39010 may be used with 545-575nm CWL LEDs.

CAL Fluor® Red 635

49015 may be used with LEDs with CWL 585-620nm.

Calcein

49011, 39002 may be used with LEDs with CWL 470-490nm; 49003 set may be used with 490-505nm LED.

Calcium Green™-1

49011, 39002 may be used with LEDs with CWL 470-490nm; 49003 set may be used with 490-505nm LED.

Calcofluor White

Calcofluor White is a commonly used stain to detect fungi and yeast and other microorganisms. It is a non-specific stain which preferentially binds to chitin and cellulose in cell walls and as such is also used to visualize plant cells. Calcofluor White has extremely broad excitation/emission spectra, and as a result almost any filter set with UV or violet/blue excitation wavelengths will generate fluorescence emission which may be detected across a very broad range. Some samples will fluoresce strongly in the green wavelengths, others in the blue, and others yellow-orange. Because of this, filter selection is subjective, partly depending on the experience of the microscopist. Our filter sets 19012, 19000, and 19011 may be used successfully depending on the sample and the observer’s preference. 19000 and 19012 may be used with LEDs with CWL 365-380nm; 19011 may be used with 395-410nm CWL LEDs.

Cerulean

49001, 39001 may be used with LEDs with CWL 430-440nm.

CFP

49001, 39001 may be used with LEDs with CWL 430-440nm.

Citrine

49003, 39003 may be used with LEDs with CWL 490-505nm.

Coumarin

49000 may be used with LEDs with CWL 350-365nm; 39000 may be used with 365-380nm CWL LEDs, 19011 may be used with 395-410nm CWL LEDs.

Cy2™

49011, 39002 may be used with LEDs with CWL 470-490nm; 49003 set may be used with 490-505nm LEDs.

Cy3.5™

49031 may be used with LEDs with CWL from 560-580nm; 49008, 39010 may be used with 545-575nm LEDs.

Cy3™

49004, 49005 may be used with LEDs with CWL 535-555nm; 39004 may be used with 535-550nm CWL LEDs.

Cy5.5™

49022 may be used with LEDs with CWL 635-665nm.

Cy5™

49006 may be used with LEDs with CWL 595-640nm; 39007 may be used with 600-635nm CWL LEDs; 49009 may be used with 625-650nm CWL LEDs.

Cy7™

49007 may be used with LEDs with CWL 685-740nm.

DAPI

49000 may be used with LEDs with CWL 350-365nm; 39000 may be used with 365-380nm CWL LEDs, 49028 may be used with 385-400nm CWL LEDs.

Di-8-ANEPPS non-ratiometric

39009 may be used with LEDs with CWL 470-490nm.

Di-8-ANEPPS, ratiometric

This dye and the related dye, Di-4-ANEPPS, are most effectively used as ratiometric indicators of membrane potential. Increases in membrane potential are indicated by a decrease in fluorescence intensity with approx. 440nm excitation and an increase in fluorescence intensity with approx. 530nm excitation. Use of this 71006 filter set requires an external filter wheel to house the 2 excitation filters and to facilitate rapid switching of filters for ratiometric measurements.

DiA

49024 may be used with LEDs with CWL 425-455nm; 39009, 19010 may be used with 470-490nm CWL LEDs.

DiD

49006 may be used with LEDs with CWL 595-640nm; 39007 may be used with 600-635nm CWL LEDs.

DiI

49004, 49305 may be used with LEDs with CWL 535-555nm; 39004 may be used with 535-550nm CWL LEDs.

DiO

49002 may be used with LEDs with CWL 455-485nm; 49020 may be used with 475-485nm CWL LEDs; 39002 may be used with 470-490nm CWL LEDs.

DiR

49007 may be used with LEDs with CWL 685-740nm.

Draq5

49006 may be used with LEDs with CWL 595-640nm; 39007 may be used with 600-635nm CWL LEDs.

DsRed

49004 may be used with LEDs with CWL 535-555nm; 39004 may be used with 535-550nm CWL LEDs.

DyLight 350

49000 may be used with LEDs with CWL 350-365nm; 39000 may be used with 365-380nm CWL LEDs.

DyLight 405

49021 may be used with LEDs with CWL 400-410nm; 19011 may be used with 395-410nm CWL LEDs.

DyLight 488

49011, 39002 may be used with LEDs with CWL 465-495nm; 49002 may be used with 455-485nm CWL LEDs.

DyLight 549

49004, 49305 may be used with LEDs with CWL 535-555nm; 39004 may be used with 535-550nm CWL LEDs.

DyLight 594

49008, 39010 may be used with LEDs with CWL 545-575nm; 49306 may be used with 570-595nm CWL LEDs.

DyLight 633

49015 may be used with LEDs with CWL 585-620nm.

DyLight 649

49006 may be used with LEDs with CWL 595-640nm; 39007 may be used with 600-635nm CWL LEDs; 49009 may be used with 625-650nm CWL LEDs.

DyLight 680

49022 may be used with LEDs with CWL 635-665nm.

DyLight 750

49007 may be used with LEDs with CWL 685-740nm.

DyLight 800

49037 may be used with LEDs with CWL 725-755nm.

EBFP2

49028 may be used with LEDs with CWL 385-400nm, 49021 may be used with 400-410nm CWL LEDs.

ECFP

49001, 39001 may be used with LEDs with CWL 430-440nm.

EGFP

49002 may be used with LEDs with CWL 455-485nm; 49020 may be used with 475-485nm CWL LEDs; 39002 may be used with 470-490nm CWL LEDs.

Emerald GFP

49002 may be used with LEDs with CWL 455-485nm; 49020 may be used with 475-485nm CWL LEDs; 39002 may be used with 470-490nm CWL LEDs.

Eosin

49023 may be used with LEDs with CWL 490-520nm.

Ethidium Bromide

49005 may be used with LEDs with CWL 535-555nm; 39009 may be used with 470-490nm CWL LEDs.

Ethidium homidimer-1/DNA

49005 may be used with LEDs with CWL 535-555nm; 39005 may be used with 535-550nm CWL LEDs.

EYFP/pH 7

49003, 39003 may be used with LEDs with CWL 490-505nm.

FAM

49011, 39002 may be used with LEDs with CWL 465-495nm; 49002 may be used with 455-485nm CWL LEDs.

FITC

49011, 39002 may be used with LEDs with CWL 465-495nm; 49002 may be used with 455-485nm CWL LEDs.

FlAsH-CCPFCC

49003, 39003 may be used with LEDs with CWL 490-505nm.

Fluo-3

49003 may be used with LEDs with CWL 490-505nm; 49011 may be used with 465-495nm CWL LEDs.

Fluo-4

49011, 39002 may be used with LEDs with CWL 465-495nm; 49002 may be used with 455-485nm CWL LEDs.

FluoroGold

This dye has mainly been used as a retrograde neuronal tracer, although it can also distinguish between DNA and RNA binding according to its fluorescence emission. With two excitation peaks at approx. 330nm and 390nm, and two emission peaks at approx. 450nm and 600nm, our DAPI longpass filter sets 49025 and 19012 are appropriate for detection of the fluorescence emission associated with the both peaks, which together appear “gold”. The 49025 set is also useful for distinguishing between FluoroGold bound to DNA or RNA.

FM™ 1-43

39009, 19010 may be used with LEDs with CWL 470-490nm.

FM™ 4-64

For imaging applications using a monochrome camera, this longpass filter set will not distinguish FM 4-64 from TRITC or similar orange/red fluorochromes. To be able to distinguish between FM 4-64 and TRITC or Texas Red-like fluorochromes, contact us for a custom filter set. 19010 may be used with LEDs with CWL 470-490nm; 19006 may be used with 545-575nm CWL LEDs.

Fura Red/Ca2+ – saturated

Fura Red/Ca2+ -free

Fura-2/Ca2+ – free

Fura-2/Ca2+ – saturated

FusionRed

49008, 39010 and 49017 may be used with LEDs with CWL 545-575nm.

GFP

EGFP spectra used here; similar to Emerald GFP, TagGFP, Azami Green, mWasabi, etc.49002 may be used with LEDs with CWL 455-485nm; 49020 may be used with 475-485nm CWL LEDs; 39002 may be used with 470-490nm CWL LEDs.

HEX,SE

49014 may be used with LEDs with CWL520-540nm; 49023 may be use with 485-515nm CWL LEDs.

Hoechst 33258

49000 may be used with LEDs with CWL 350-365nm; 39000 may be used with 365-380nm CWL LEDs.

Indo-1/Ca2+ -free

79006 may be used with LEDs with CWL 365nm.

Indo-1/Ca2+ -saturated

79006 may be used with LEDs with CWL 365nm.

Indocyanine Green

The spectral properties of ICG are strongly dependent on its concentration and the solute it is bound to, so care must be taken by workers to characterize their particular model system. This filter set 49030 is appropriate for most applications involving protein-bound ICG in various physiological media. ICG becomes concentrated in lipid environments, greatly shifting its spectral properties. 49030 may be used with LEDs with CWL 755-795nm.

JC-1 non-ratiometric

JC-1 exists as a monomer at low concentrations and/or low membrane potentials and displays fluorescence ex./em. maxima similar to that of YFP. At higher concentrations and/or higher membrane potentials, JC-1 forms aggregates with a red-shifted emission maxima of 590nm and a broadened excitation spectra. The 49012 longpass filter set will allow detection of JC-1 monomer and aggregate simultaneously. 49012 may be used with LEDs with CWL 465-495nm; 19002 may be used with 470-490nm CWL LEDs.

JC-1 ratiometric

JC-1 exists as a monomer at low concentrations and/or low membrane potentials and displays fluorescence ex./em. maxima similar to that of YFP. At higher concentrations and/or higher membrane potentials, JC-1 forms aggregates with a red-shifted emission maxima of 590nm and a broadened excitation spectra. Use of this 71019 filter set requires an external filter wheel to house the 2 emission filters to facilitate rapid switching of filters for ratiometric measurements.

JOE

49023 may be use with LEDs with CWL 485-515nm.

Killer Red

49008, 39010 may be used with LEDs with CWL 545-575nm; 49306 may be used with 570-595nm CWL LEDs.

Li-Cor IRDye® 680LT

49022 may be used with LEDs with CWL 635-665nm.

Li-Cor IRDye® 800CW

Lucifer Yellow

49026 may be used with LEDs with CWL 390-415nm; 39008 may be used with 405-430nm CWL LEDs.

LysoTracker Blue/MeOH

49028 may be used with LEDs with CWL 385-400nm; 49000 may be used with LEDs with CWL 350-365nm; 39000 may be used with 365-380nm CWL LEDs.

LysoTracker Green/pH 5.2

49002 may be used with LEDs with CWL 455-485nm; 49020 may be used with 475-485nm CWL LEDs; 39002 may be used with 470-490nm CWL LEDs.

LysoTracker Red/pH 5.2

49008 may be used with LEDs with CWL 545-575nm; 49305 may be used with 535-550nm CWL LEDs.

LysoTracker Yellow HCK-123

49023 may be used with LEDs with CWL 485-515nm; 39009 may be use with 470-490nm CWL LEDs.

mCherry

49008, 39010 may be used with LEDs with CWL 545-575nm; 49306 may be used with 570-595nm CWL LEDs.

mCitrine

49003, 39003 may be used with LEDs with CWL 490-505nm; 49023 may be use with 490-520nm CWL LEDs.

MitoTracker Deep Red 633/MeOH

49006 may be used with LEDs with CWL 595-640nm; 39007 may be used with 600-635nm CWL LEDs; 49009 may be used with 625-650nm CWL LEDs.

MitoTracker Green FM/MeOH

49002 may be used with LEDs with CWL 455-485nm; 49011 may be used with 465-495nm CWL LEDs; 39002 may be used with 470-490nm CWL LEDs.

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MitoTracker Orange/MeOH

49004, 49305 may be used with LEDs with CWL 535-555nm; 39004 may be used with 535-550nm CWL LEDs.

MitoTracker Red/MeOH

49008, 39010 may be used with LEDs with CWL 545-575nm; 49306 may be used with 570-595nm CWL LEDs.

mKate2

49008, 39010 may be used with LEDs with CWL 545-575nm; 49306 may be used with 570-595nm CWL LEDs.

mKeima Red

49024 may be used with LEDs with CWL 430-450nm.

mKO

49014 may be used with LEDs with CWL 520-540nm; 49010 is not appropriate for use with LEDs because the bandwidth is too narrow.

mOrange2

49014 may be used with LEDs with CWL 520-540nm; 49010 is not appropriate for use with LEDs because the bandwidth is too narrow.

mPlum

49017, 19006 may be used with LEDs with CWL 545-575nm

mRFP1

49008, 39010 may be used with LEDs with CWL 545-575nm; 49306 may be used with 570-595nm CWL LEDs.

mTFP1

49013 may be used with LEDs with CWL 435-455nm.

mWasabi

49002 may be used with LEDs with CWL 455-485nm; 49020 may be used with 475-485nm CWL LEDs; 39002 may be used with 470-490nm CWL LEDs.

NBD X/MeOH

49011 may be used with LEDs with CWL 465-495nm; 19008 may be used with 435-465nm CWL LEDs.

Nile Blue

49006 may be used with LEDs with CWL 595-640nm; 39007 may be used with 600-635nm CWL LEDs.

NirFP

49019 may be used with LEDs with CWL 595-640nm.

Oregon Green™ 488

49011, 39002 may be used with LEDs with CWL 470-490nm; 49003 set may be used with 490-505nm LED.

Oregon Green™ 514

49003, 39003 may be used with LEDs with CWL 490-505nm. 49303 may be used with 490-505nm CWL LEDs.

Pacific Blue

49021 may be used with LEDs with CWL 400-410nm; 19011 may be used with 395-410nm CWL LEDs.

Propidium Iodide

49005 may be used with LEDs with CWL 535-555nm; 39005 may be used with 535-550nm CWL LEDs.

Pulsar™ 650

49024 may be used with LEDs with CWL 430-450nm; 39009, 19010 may be used with 470-490nm CWL LEDs.

Qdot 525

Although the excitation maxima of qdot nanocrystals is in the UV, a broad range of excitation wavelengths may be used.

Qdot 545

Although the excitation maxima of qdot nanocrystals is in the UV, a broad range of excitation wavelengths may be used.

Qdot 565

Although the excitation maxima of qdot nanocrystals is in the UV, a broad range of excitation wavelengths may be used.

Qdot 585

Although the excitation maxima of qdot nanocrystals is in the UV, a broad range of excitation wavelengths may be used.

Qdot 605

Although the excitation maxima of qdot nanocrystals is in the UV, a broad range of excitation wavelengths may be used.

Qdot 625

Although the excitation maxima of qdot nanocrystals is in the UV, a broad range of excitation wavelengths may be used.

Qdot 655

Although the excitation maxima of qdot nanocrystals is in the UV, a broad range of excitation wavelengths may be used.

Qdot 705

Although the excitation maxima of qdot nanocrystals is in the UV, a broad range of excitation wavelengths may be used.

Qdot 800

Although the excitation maxima of qdot nanocrystals is in the UV, a broad range of excitation wavelengths may be used.

Quasar® 570

49014 may be used with LEDs with CWL 520-540nm; 49304 may be used with 540-550nm CWL LEDs.

Quasar® 670

49006 may be used with LEDs with CWL 595-640nm; 39007 may be used with 600-635nm CWL LEDs; 49009 may be used with 625-650nm CWL LEDs.

Quasar® 705

49022 may be used with LEDs with CWL 635-670nm.

R-phycoerythrin

49014 may be used with LEDs with CWL 520-540nm; 19004 may be used with 535-545nm CWL LEDs; 49010 is not appropriate for use with LEDs because the bandwidth is too narrow.

ReAsH-CCPGCC

49008, 39010 may be used with LEDs with CWL 545-575nm; 49306 may be used with 570-595nm CWL LEDs.

Resorufin

49004, 49305 may be used with LEDs with CWL 535-555nm; 39004 may be used with 535-550nm CWL LEDs.

Rhod-2

49004, 49305 may be used with LEDs with CWL 535-555nm; 39004 may be used with 535-550nm CWL LEDs.

Rhodamine 123

49003, 39003 may be used with LEDs with CWL 490-505nm.

Rhodamine 6G

49023 may be use with LEDs with CWL 485-515nm.

Rhodamine Red™-X

49008, 39010 may be used with LEDs with CWL 545-575nm.

ROX

49008, 39010 may be used with LEDs with CWL 545-575nm.

SBFI/Na+ -free

SBFI/Na+ -saturated

SNARF pH 6.0

SNARF pH 9.0

Sulforhodamine 101

49008, 39010 may be used with LEDs with CWL 545-575nm.

SYBR® Green I

49011, 39002 may be used with LEDs with CWL 465-495nm; 49002 may be used with 455-485nm CWL LEDs.

SYTO 9/DNA

49011, 39002 may be used with LEDs with CWL 465-495nm; 49002 may be used with 455-485nm CWL LEDs.

SYTO® 60

49006 may be used with LEDs with CWL 595-640nm; 39007 may be used with 600-635nm CWL LEDs; 49009 may be used with 625-650nm CWL LEDs.

T-Sapphire

TagBFP

49021 may be used with 400-410nm CWL LEDs, 49028 may be used with 385-400nm CWL LEDs.

TagRFP

49004, 49305 may be used with LEDs with CWL 535-555nm; 39004 may be used with 535-550nm CWL LEDs.

TAMRA

49004, 49305 may be used with LEDs with CWL 535-555nm; 39004 may be used with 535-550nm CWL LEDs.

tdTomato

49004, 49305 may be used with LEDs with CWL 535-555nm; 39004 may be used with 535-550nm CWL LEDs.

TET

49023 may be used with LEDs with CWL 485-515nm.

Tetracycline

Tetramethylrhodamine isothiocyanate

49004, 49305 may be used with LEDs with CWL 535-555nm; 39004 may be used with 535-550nm CWL LEDs.

Texas Red®

49008, 39010 may be used with LEDs with CWL 545-575nm; 49306 may be used with 570-595nm CWL LEDs.

Texas Red®-X

49008, 39010 may be used with LEDs with CWL 545-575nm; 49306 may be used with 570-595nm CWL LEDs.

TO-PRO™-3

49006 may be used with LEDs with CWL 595-640nm; 39007 may be used with 600-635nm CWL LEDs; 49009 may be used with 625-650nm CWL LEDs.

Topaz

49003, 39003 may be used with LEDs with CWL 490-505nm.

TRITC

49004, 49305 may be used with LEDs with CWL 535-555nm; 39004 may be used with 535-550nm CWL LEDs.

TurboFP650

49008, 49017 and 19006 may be used with LEDs with CWL 545-575nm.

wtGFP

19008 may be used with LEDs with CWL 435-470nm; 49002 may be used with 455-485nm CWL LEDs

XTop

X-rhod-1/Ca2+

49008, 39010 may be used with LEDs with CWL 545-575nm; 49306 may be used with 570-595nm CWL LEDs.

ZsGreen1

49002 may be used with LEDs with CWL 455-485nm; 49020 may be used with 475-485nm CWL LEDs; 39002 may be used with 470-490nm CWL LEDs.

ZsYellow

49023 may be use with LEDs with CWL 485-515nm; 49003 set may be used with 490-505nm LED.

Fluorescence, Fluorochrome, Optical Fiters

JNO-Qdot625

– Filter set for Qdot625
– Chroma Filter Model No. : 39106

SpectraViewer – Qdot625
SpectraViewer - Qdot625
SpectraViewer – Qdot625

Spectrum_JNO-Qdot625

JNO-Qdot625
JNO-Qdot625
Chroma Model No. 39106
Filters in this SetType
AT425/50x EX
AT485DCBS
AT625/30mEM
JNO-Qdot625

상기 필터를 구매하시기 전에, Excitation 영역에서 사용하시는 광원이 충분한 광량을 가지고 있는지 확인을 하셔야 하고, Emission영역이 가시광선 대역을 벗어나는 경우에는 검출장치(카메라)가 대응 가능한 영역인지 확인이 필요합니다.

현미경의 종류에 따라 Filter의 Size가 다르고, 같이 사용되어 지는 Cube의 종류가 다양하기 때문에, 구매시에는 반드시 판매 담당자와 충분한 확인을 하실 필요가 있습니다.

E-mail : jhjin@jnoptic.com 
Mobile : + 82-2-3473-4188~9
AcquCAM, Camera, Fluorescence

AcquCAM Pro/G3

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AcquCAM Pro/G3

Down load Catalog _ AcquCAM PRO/G3

AcquCAM Pro/G3

 AcquCAM Pro/G3 USB 3.0 camera is the perfect solution for many bio, industrial, medical applications. Utilizing the very sensitive color CCD sensors, these cameras feature a specialization of fluorescence observations. With up to 30 fps and a trigger delay of less than 5 micro seconds.

  • 1.2 Mega pixel CCD camera
  • Color, Progressive scan
  • Preview software provided (JNOPTIC Capture)
  • Software compatible with Windows XP/ Vista/ 7/ 8 operating systems

LIVE PSEUDO COLOR

Ex. of applied PSEUDO COLOR for black live fluorescence image

Emphasis function on variety of images

AcquCAM Pro G3_15
With emphasis function, BLACK BALANCE / AUTO LEVEL of ARM on variety images, you could acquire cleaner and sharper images.

Acquiring high sensitive images

These images were shot by AcquCAM Silver Package.

Acquiring high sensitive images from general lighting source

[Specification]

SpecDescription
Size1/3 inch
ResolutionH: 1280, V: 960
Pixel sizeH: 3.75 µm, V: 3.75 µm
Video formats   & Frame rate1280×960 RGB32 @ 30, 20, 15, 7.5, 3.75 fps   1280×960 Y800 @ 30, 20, 15, 7.5, 3.75 fps 1280×960 Y16 @ 30, 20, 15, 7.5, 3.75 fps
Sensitivity0.05 lx
Exposure time1/10000 to 30 s
Lens mountC/Cs mount
InterfaceUSB 3.0
Power supply4.5 to 5.5 VDC
DimensionsH: 29 mm, W: 29 mm, L: 43 mm
Mass65 g
Camera, Fluorescence, PHYSIOLOGY

AcquCAM 23S2

AcquCAM 23S2

가시광선 & 근적외선(NIR) 관찰

CA3 pyramidal neuron IR-DIC Camera : AcquCAM 23S2
CA3 pyramidal neuron, IR-DIC & Alexa Fluo 488 at the same time
Figure courtesy by Dr. Sooyun Kim, Seoul National University
with Bx51WI, JNO-DPTS, AcquCAM 23S
JNO-DPTS
JNO-DPTS CA3 pyramidal neuron, IR-DIC & Alexa Fluo 488 at the same time Figure courtesy by Dr. Sooyun Kim, Seoul National University with Bx51WI, JNO-DPTS, AcquCAM 23S
  • USB 3.0 Monochrome Camera
  • 1/1.2 ” Pregius Sensor
  • 1920×1200 pixel
  • Trigger input and I/O
  • Cost-effective mono camera for fluorescence microscope (Best)
  • Cost-effective camera for Department of Physiology (Best)
  • 1/1.2 inch optimized sensor size for microscope field of view
  • Acquire images of optimum width combined with 0.63x adapter
Microscopy Camera
AcquCAM 23S2

AcquCAM 23S2 mono camera has a USB 3.0 interface and is the perfect solution for many industrial automation, quality assurance, security, surveillance and medical applications. The mono camera ships with the very sensitive 1/1.2 inch CMOS Pregius  sensor. With up to 162 images per second, the AcuqCAM 23S2 is a low cost, yet highly versatile imaging solution. The camera includes a C to CS mount adapter, making it compatible to C and CS mount lenses.

Images with AcquCAM 23S2
AcquCAM 23S2 Fluorescence camera DAPI, FITC, TRITC
Sample : bovine pulmonary artery endothelial cell, BPAEC Filter Set : JNO-U(B), B(B), G(B) Camera : AcquCAM 23S2 C-mount adapter : 1x Lens : UPlanAPO 40x Above three images are merged and processed using ARM S/W
AcquCAM 23S2 Fluorescence camera DAPI, FITC, TRITC
Sample : bovine pulmonary artery endothelial cell, BPAEC Filter Set : JNO-U(B), B(B), G(B) Camera : AcquCAM 23S2 C-mount adapter : 1x Lens : UPlanAPO 40x Above three images are merged and processed using ARM S/W

Specification

SpecDescription
  Size  1/1.2 inch
  Resolution  H: 1920, V: 1200
 Pixel size  H: 5.86 µm, V: 5.86 µm
 Video formats & Frame rate1920×1200  Y16    @    80 fps
1920×1200  Y800 @ 162 fps
  Sensitivity  0.015 lx
  Exposure time  20㎲ to 30 s
  Lens mount  C/Cs mount
  Interface  USB 3.0
  Power supply  4.5 to 5.5 VDC
  Dimensions  H: 29 mm, W: 29 mm, L: 43 mm
  Mass  65 g

AcquCAM 카메라 현미경 관찰법 대응표

AcquCAM 카메라 스펙 비교표

Setup Driver & Software Download

AcquCAM, Camera, Fluorescence

AcquCAM 3G

댓글 한 개

AcquCAM 3G

  • USB 3.0 Color Camera
  • 1/1.8 ” Pregius Sensor
  • 2048 x 1536 pixel
  • Trigger input and I/O
  • Cost-effective color camera
  • High Sensitivity Sensor
  • Fast, seamless image transfer
  • Available of images with a very wide field of view
  • Low Noise Sensor
AcquCAM 3G
AcquCAM 3G

AcquCAM 3G color camera has a USB 3.0 interface and is the perfect solution for many industrial automation, quality assurance, security, surveillance and medical applications. The color camera ships with the very sensitive 1/1.8 inch CMOS Pregius  sensor. With up to 60 images per second, the AcuqCAM 3G is a low cost, yet highly versatile imaging solution. The camera includes a C to CS mount adapter, making it compatible to C and CS mount lenses. Using the optional CS to M12 board lens adapter, the camera is
also compatible to M12 board lenses.

Images with AcquCAM 3G

DAPI Sample : bovine pulmonary artery endothelial cell, BPAEC Camera : AcquCAM 3G, Filter Set : JNO-U(B)
DAPI
Filter Set : JNO-U(B)
FITC Sample : bovine pulmonary artery endothelial cell, BPAEC Camera : AcquCAM 3G, Filter Set : JNO-B(B)
FITC
Filter Set : JNO-B(B)
TRITC Sample : bovine pulmonary artery endothelial cell, BPAEC Camera : AcquCAM 3G, Filter Set : JNO-U(B),B(B),G(B)
TRITC
Filter Set : JNO-G(B)
Sample : bovine pulmonary artery endothelial cell, BPAEC Camera : AcquCAM 3G, Filter Set : JNO-U(B),B(B),G(B) Objective lens : UPLNAPO40X, C-mount adapter : 1X Above three images are merged and processed using JNO-ARM
Sample : bovine pulmonary artery endothelial cell, BPAEC Camera : AcquCAM 3G, Filter Set : JNO-U(B),B(B),G(B) Objective lens : UPLNAPO40X, C-mount adapter : 1X Above three images are merged and processed using JNO-ARM

Specification

SpecDescription
Size1/1.8 inch
ResolutionH: 2048, V: 1536
Pixel sizeH: 3.45 µm, V: 3.45 µm
Video formats& Frame rate2048×1536 (3.1 MP) RGB64 @ 60 fps   2048×1536 (3.1 MP) RGB32 @ 60 fps
2048×1536 (3.1 MP) Y16 @ 60 fps 2048×1536 (3.1 MP) Y411 @ 60 fps
2048×1536 (3.1 MP) YUY2 @ 60 fps
Dynamic range8 / 12 bit
Exposure time20 ㎲ to 30s
Lens mountC/Cs mount
InterfaceUSB 3.0
Power supply4.5 to 5.5 VDC
DimensionsH: 29 mm, W: 29 mm, L: 43 mm
Mass65g

AcquCAM 카메라 현미경 관찰법 대응표

AcquCAM 카메라 스펙 비교표

Setup Driver & Software Download

Fluorochrome

Fluorochrome – Cy7™

Cy7™

Spectrum Chart

Cy7 Spectrum
Excitation (㎚)Emission (㎚)
743767

Recommended Filter set

  • 일반 형광 Lighting source
Model No.ExcitationDichroic mirrorEmissionRemarks
JNO-49007ET710/75xT760lpxrET810/90m–
JNO-49037ET740/40xT770lpxrET780lp–

Cy7 & JNO-49007 Specrtum Chart for CoollED pE4000
Cy7 & JNO-49037 Specrtum Chart for CoollED pE4000
  • Cy7을 관찰하기 위한 형광 광원 장치
    • T. 02-3473-4188로 연락 주시기 바랍니다.

Information

Cyanines were and are still used in industry, and more recently in biotechnology (labeling, analysis). Cyanines have many uses as fluorescent dyes, particularly in biomedical imaging. Depending on the structure, they cover the spectrum from IR to UV.

Cyanine is the non-systematic name of a synthetic dye family belonging to polymethine group. The word cyanin is from the English word “cyan”, which conventionally means a shade of blue-green (close to “aqua”) and is derived from the Greek κυάνεος/κυανοῦς kyaneos/kyanous which means a somewhat different color: “dark blue”.

Cy7 is a near-IR fluor that is invisible to the naked eye (Excitation/emission maximum 750/776 ㎚). It is used in in vivo imaging applications, as well as the Cy7.5 dye.

Cyanines(Cy) replace advantageously conventional dyes such as Fluorescein(FITC) and rhodamines (TRITC, RRX), yielding brighter and more stable fluorescence.

시아닌은 여전히 업계에서, 그리고 최근에는 생명 공학 (라벨링, 분석)에서 사용되고 있습니다. 시아닌은 형광 염료로서, 특히 생체 의학 이미징에서 많은 용도로 사용되며 구조에 따라 IR에서 UV까지 스펙트럼을 커버합니다.

Cyanine은 polymethine 그룹에 속하는 합성 염료 계열의 비 계통적 이름입니다. cyanin이라는 단어는 일반적으로 청록색 ( “아쿠아”에 가까운)의 그늘을 의미하는 영어 단어 “cyan”에서 유래되었으며 다소 다른 색상 “진한 파란색 “을 의미하는 그리스어 κυάνεος / κυανοῦς kyaneos / kyanous에서 파생되었습니다.

Cy7은 육안으로는 보이지 않는 근적외선 형광체이며, Cy7.5 염료와 함께 생체 내 이미징 어플리케이션에 사용됩니다.

Cyanines (Cy)는 Fluorescein (FITC) 및 rhodamines (TRITC, RRX)와 같은 기존의 염료를 대체하여 보다 밝고 안정한 형광을 생성합니다.

Fluorescence, MICROSCOPY

Fluorescent Samples

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판매용 형광 샘플

Fluorescent Samples for Sale

이미지 상단부분을 클릭하시면 원본 이미지를 확인하실 수 있습니다.

판매용 형광 샘플 (U&B자극)
U & B 자극
판매용 형광 샘플 (U&G&R자극)
U & G & R 자극
판매용 형광 샘플 (B&G&R자극)
B & G & R 자극
판매용 형광 샘플 ((G&R자극)
G & R 자극
판매용 형광 샘플 (B자극)
B 자극
판매용 형광 샘플 (U자극)
U 자극
판매용 형광 샘플(U&B&G&R자극)
판매용 형광 샘플
-U & B & G & R자극 –

형광샘플 촬영 조건

  • 4ch 크로마 형광 필터
  • Camera: AcquCAM 23GR2
  • Objetives: UPlanApo40x
  • Camera Adapter: 1x
  • Light source: CoolLED pE-300ultra

Fluorescence

Fluorophores

Browse By Fluorochrome

Compare filter sets by selecting the recommended/alternative set listed for each fluorochrome below.

  • If you need more information, please click fluorochrome. You can see each fluorochrome information and recommended filter sets.
  • If you need other dye information, please contact us.
  • Tel : 02-3473-4188~9
  • E-mail : jhjin@jnoptic.com

하단의 Fluorochrome 이름을 클릭하시면
대응되는 형광필터의 조합을 확인 하실 수 있습니다.

AlexaFluor 594

Alexa Fluor 594 dye is a bright, red-fluorescent dye that can be excited using the 561 nm or 594 nm laser lines. For stable signal generation in imaging and flow cytometry, Alexa Fluor 594 dye is pH-insensitive over a wide molar range.

Spectrum-Chart_Alexa-Fluor-594
Spectrum-Chart_Alexa-Fluor-594

CFP

Cyan Fluorescent Protein (CFP) is a versatile biological marker for monitoring physiological processes, visualizing protein localization, and detecting transgenic expression in vivo. CFP can be excited by the 405 nm laser line and is optimally detected at 485 nm.

Spectrum-Chart_CFP
Spectrum-Chart_CFP

Cy5

Cy5 dye is a bright, far-red-fluorescent dye that can be excited using the 633 or 647 nm laser lines, and visualized Cy5 filter sets.  In addition to immunocytochemistry applications, Cy5 is also commonly used to label nucleic acids.

Spectrum-Chart_Cy5
Spectrum-Chart_Cy5

Cy5.5

A variety of cyanine 5.5 (Cy5.5) dyes has been used to label biological molecules for fluorescence imaging and other fluorescence-based biochemical analysis. They are widely used for labeling peptides, proteins and oligos etc. Cy5.5 dyes are one type of the most common red fluorophores.

Spectrum-Chart_Cy5.5
Spectrum-Chart_Cy5.5

Cy7

Cy7 is a near-IR fluor that is invisible to the naked eye (Excitation/emission maximum 750/776 nm). It is used in in vivo imaging applications, as well as the Cy7.5 dye.

Spectrum-Chart_Cy7
Spectrum-Chart_Cy7

DAPI

DAPI (4′,6-diamidino-2-phenylindole) is a fluorescent stain that binds strongly to A-T rich regions in DNA. It is used extensively in fluorescence microscopy. As DAPI can pass through an intact cell membrane, it can be used to stain both live and fixed cells, though it passes through the membrane less efficiently in live cells and therefore the effectiveness of the stain is lower.

Spectrum-Chart_DAPI
Spectrum-Chart_DAPI

FITC

Fluorescein isothiocyanate (FITC) is a derivative of fluorescein used in wide-ranging applications including flow cytometry. FITC is the original fluoresce in molecule functionalized with an isothiocyanate reactive group (-N=C=S), replacing a hydrogen atom on the bottom ring of the structure. This derivative is reactive towards nucleophiles including amine and sulfhydryl groups on proteins.

Spectrum-Chart_FITC
Spectrum-Chart_FITC

GFP

The green fluorescent protein (GFP) is a protein composed of 238 amino acid residues (26.9 kDa) that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. Although many other marine organisms have similar green fluorescent proteins, GFP traditionally refers to the protein first isolated from the jellyfish Aequorea victoria. The GFP from A. victoria has a major excitation peak at a wavelength of 395 nm and a minor one at 475 nm. Its emission peak is at 509 nm, which is in the lower green portion of the visible spectrum.

Spectrum-Chart_GFP
Spectrum-Chart_GFP

tdTomato

tdTomato is a genetic fusion of two copies of the dTomato gene (2) which was specifi cally designed for low aggregation (1). Its tandem dimer structure plays an important role in the exceptional brightness of tdTomato (Table I). Its excitation and emission maxima occur at 554 nm and 581 nm, respectively (1). Because tdTomato forms an intramolecular dimer, it behaves like a monomer, and has been used successfully for N- and C- terminal fusions. It shows excellent photostability and its half-time (t0.5) for maturation is one hour at 37°C.

TRITC

Tetramethylrhodamine (TRITC) is a bright orange-fluorescent dye with excitation ideally suited to the 532 nm laser line. It is commonly conjugated to antibodies and proteins for cellular imaging applications.

Spectrum-Chart_TRITC
Spectrum-Chart_TRITC