카테고리 보관물: Fluorescence

JNO-G(L)

2018년 1월 18일 jhjin

JNOPTIC Filter JNO-G(L)

– Filter set for TRITC / Cy3 Longpass
– Chroma Filter Model No. : 19004

JNO-G(L) Spectrum

Standard Green Excitaion filter (Long Pass type)

Filters	Type
AT540/25x	EX
AT565DC	BS
AT575lp	EM

주요 형광 색소(Fluorochromes)와 스펙트럼 비교

* TRITC VS JNO-G(L)

JNO-G(L)필터를 이용한 Sample Image 촬영

Fluorescence image taken using JNO-G(L) — Filter Set : JNO-G(L) Camera : AcquCAM 23GR2 C-mount adapter : 1x Lens : UPLAPO40X Sample : bovine pulmonary artery endothelial cell, BPAEC

JNO-G(L)필터에 적합한 형광색소

R-phycoerythrin

JNO-G(L) Spec

Typical Application(s) : Widefield Microscopy
Coating : Sputter/Hard Coated
Round excitation and emission filters mounted in anodized aluminum rings 2.3mm thick, up to 25mm in diameter (for standard sizes)
Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes (for standard sizes)

*** For filters larger than 25mm and dichroics larger than 26x38mm, please contact us for pricing.

CHROMA, Fluorescence

JNO-B(L)

2018년 1월 18일 jhjin

JNOPTIC Filter JNO-B(L)

– Filter set for GFP / FITC Longpass
– Chroma Filter Model No. : 19002

JNO-B(L) Spectrum

Filters	Type
AT480/30x	EX
AT505DC	BS
AT515lp	EM

주요 형광 색소(Fluorochromes)와 스펙트럼 비교

* FITC VS JNO-B(L)

* GFP VS JNO-B(L)

JNO-B(L)필터를 이용한 Sample Image 촬영

Fluorescence image taken using JNO-B(L) — Sample : bovine pulmonary artery endothelial cell, BPAEC Filter Set : JNO-B(L) Camera : AcquCAM 23GR2 C-mount adapter : 1X Lens : UPLNAPO40X

JNO-B(L)필터에 적합한 형광색소

Acridine Orange + DNA and RNA
Auramine-rhodamine
JC-1 non-ratiometric

JNO-B(L) Spec

Typical Application(s) : Widefield Microscopy
Coating : Sputter/Hard Coated
Round excitation and emission filters mounted in anodized aluminum rings 2.3mm thick, up to 25mm in diameter (for standard sizes)
Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes (for standard sizes)

*** For filters larger than 25mm and dichroics larger than 26x38mm, please contact us for pricing. 02-3473-4188~9

CHROMA, Fluorescence

JNO-R2(B)

2018년 1월 18일 jhjin

JNOPTIC Filter JNO-R2(B)

– Filter set for Cy5 / AlexaFluor 647 / Draq5
– Chroma Filter Model No. : 39007

JNO-R2(B) Spectrum

Filters	Type
AT620/50x	EX
AT655DC	BS
AT690/50m	EM

주요 형광 색소(Fluorochromes)와 스펙트럼 비교

* Cy5 VS JNO-R2(B)

* AlexaFluor 647 VS JNO-R2(B)

* Draq5 VS JNO-R2 (B)

JNO-R2(B)필터에 적합한 형광색소

Alexa Fluor 647™
Atto 647N
Cy5™
DiD
Draq5
DyLight 649
MitoTracker Deep Red 633/MeOH
Nile Blue
Quasar® 670
SYTO® 60
TO-PRO™-3

JNO-R2(B) Spec

Typical Application(s) : Widefield Microscopy
Coating : Sputter/Hard Coated
Round excitation and emission filters mounted in anodized aluminum rings 2.3mm thick, up to 25mm in diameter (for standard sizes)
Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes (for standard sizes)

*** For filters larger than 25mm and dichroics larger than 26x38mm, please contact us for pricing.

CHROMA, Fluorescence

JNO-R1(B)

2018년 1월 18일 jhjin

JNOPTIC Filter JNO-R1(B)

– Filter set for Texas Red / mCherry / AlexaFluor 594
– Chroma Filter Model No. : 39010

JNO-R1(B) Spectrum

Filters	Type
AT560/40x	EX
AT600DC	BS
AT635/60m	EM

주요 형광 색소(Fluorochromes)와 스펙트럼 비교

* AlexaFluor 594 VS JNO-R1(B)

* Texas Red VS JNO-R1(B)

* mCherry VS JNO-R1(B)

JNO-R1(B)필터에 적합한 형광색소

Alexa Fluor 568™
Alexa Fluor 594™
CAL Fluor® Red 610
Cy3.5™
DyLight 594
FusionRed
Killer Red
mCherry
MitoTracker Red/MeOH
mKate2
mRFP1
ReAsH-CCPGCC
Rhodamine Red™-X
ROX
Sulforhodamine 101
Texas Red®
Texas Red®-X
X-rhod-1/Ca2+

JNO-R1(B) Spec

Typical Application(s) : Widefield Microscopy
Coating : Sputter/Hard Coated
Round excitation and emission filters mounted in anodized aluminum rings 2.3mm thick, up to 25mm in diameter (for standard sizes)
Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes (for standard sizes)

*** For filters larger than 25mm and dichroics larger than 26x38mm, please contact us for pricing.

CHROMA, Fluorescence

JNO-G(B)

2018년 1월 18일 jhjin

JNOPTIC Filter JNO-G(B)

– Filter set for TRITC / Cy3 / TagRFP / AlexaFluor546
– Chroma Filter Model No. : 39004

JNO-G(B) Spectrum

Filters	Type
AT540/25x	EX
AT565DC	BS
AT605/55m	EM

주요 형광 색소(Fluorochromes)와 스펙트럼 비교

* TRITC VS JNO-G(B)

* Cy3 VS JNO-G(B)

* AlexaFluor546 VS JNO-G(B)

JNO-G(B)필터를 이용한 Sample Image 촬영

Fluorescence image taken using JNO-G(B) — Filter Set : JNO-G(B) Camera : AcquCAM 3G C-mount adapter : 1x Lens : UPLAPO20X Sample : bovine pulmonary artery endothelial cell, BPAEC

JNO-G(B)필터에 적합한 형광색소

Alexa Fluor 546™
Alexa Fluor 555™
Atto 550
CAL Fluor® Red 590
Cy3™
DiI
DsRed
DyLight 549
MitoTracker Orange/MeOH
Resorufin
Rhod-2
TagRFP
TAMRA
tdTomato
Tetramethylrhodamine isothiocyanate
TRITC

JNO-G(B) Spec

Typical Application(s) : Widefield Microscopy
Coating : Sputter/Hard Coated
Round excitation and emission filters mounted in anodized aluminum rings 2.3mm thick, up to 25mm in diameter (for standard sizes)
Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes (for standard sizes)

*** For filters larger than 25mm and dichroics larger than 26x38mm, please contact us for pricing.

CHROMA, Fluorescence

JNO-B(B)

2018년 1월 18일 jhjin

JNOPTIC Filter JNO-B(B)

– Filter set for EGFP / FITC / Cy2 / AlexaFluor488
– Chroma Filter Model No. : 39002

JNO-B(B) Spectrum

Filters	Type
AT480/30x	EX
AT505DC	BS
AT535/40m	EM

주요 형광 색소(Fluorochromes)와 스펙트럼 비교

* FITC VS JNO-B(B)

* GFP VS JNO-B(B)

* AlexaFluor488 VS JNO-B(B)

JNO-B(B)필터를 이용한 Sample Image 촬영

Fluorescence image taken using JNO-B(B) — Sample : bovine pulmonary artery endothelial cell, BPAEC Filter Set : JNO-B(B) Camera : AcquCAM 23GR2 C-mount adapter : 1X Lens : PlanApo60xWLSM

JNO-B(B)필터에 적합한 형광색소

Acridine Orange + DNA
Alexa Fluor 488™
Atto 488
Azami Green
BODIPY FL/pH7.2
Calcein
Calcium Green™-1
Cy2™
DiO
DyLight 488
EGFP
Emerald GFP
FAM
FITC
Fluo-4
GFP
LysoTracker Green/pH 5.2
MitoTracker Green FM/MeOH
mWasabi
Oregon Green™ 488
SYBR® Green I
SYTO 9/DNA
ZsGreen1

JNO-B(B) Spec

Typical Application(s) : Widefield Microscopy
Coating : Sputter/Hard Coated
Round excitation and emission filters mounted in anodized aluminum rings 2.3mm thick, up to 25mm in diameter (for standard sizes)
Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes (for standard sizes)

*** For filters larger than 25mm and dichroics larger than 26x38mm, please contact us for pricing. ( Tel. 02-3473-4188)

CHROMA, Fluorescence

JNO-U(B)

2018년 1월 18일 jhjin

– Filter set for DAPI / Hoechst / AlexaFluor350
– Chroma Filter Model No. : 39000

JNO-U(B) Spectrum

Filters	Type
AT375/28x	EX
AT415DC	BS
AT460/50m	EM

주요 형광 색소(Fluorochromes)와 스펙트럼 비교

* DAPI VS JNO-U(B)

* Hoechst VS JNO-U(B)

JNO-U(B) VS Hoechst — Spectrum Comparison by Wavelength JNO-U(B) vs Hoechst

* AlexaFluor350 VS JNO-U(B)

Spectrum Comparison by Wavelength JNO-U(B) vs AF350

JNO-U(B)필터를 이용한 Sample Image 촬영

DAPI Sample : bovine pulmonary artery endothelial cell, BPAEC Camera : AcquCAM 23GR2, Filter Set : JNO-U(B) Objective lens : UPLNAPO40X, C-mount adapter : 1X — DAPI
Sample : bovine pulmonary artery endothelial cell, BPAEC
Camera : AcquCAM 23GR2, Filter Set : JNO-U(B)
Objective lens : UPLNAPO40X, C-mount adapter : 1X

DAPI, FITC, TRITC Sample : bovine pulmonary artery endothelial cell, BPAEC Camera : AcquCAM 23GR2, Filter Set : JNO-U(B),B(B),G(B) Objective lens : UPLNAPO40X, C-mount adapter : 1X (OLYMPUS) Above three images are merged and processed using JNO-ARM — DAPI, FITC, TRITC
Sample : bovine pulmonary artery endothelial cell, BPAEC
Camera : AcquCAM 23GR2, Filter Set : JNO-U(B),B(B),G(B)
Objective lens : UPLNAPO40X, C-mount adapter : 1X (OLYMPUS)
Above three images are merged and processed using JNO-ARM

DAPI, FITC, TRITC Sample : bovine pulmonary artery endothelial cell, BPAEC Camera : AcquCAM 3G, Filter Set : JNO-U(B),B(B),G(B) Objective lens : UPLNAPO40X, C-mount adapter : 1X Above three images are merged and processed using JNO-ARM — DAPI, FITC, TRITC
Sample : bovine pulmonary artery endothelial cell, BPAEC
Camera : AcquCAM 3G, Filter Set : JNO-U(B),B(B),G(B)
Objective lens : UPLNAPO40X, C-mount adapter : 1X
Above three images are merged and processed using JNO-ARM

JNO-U(B) 필터에 적합한 형광색소

Alexa Fluor 350™
Biosearch Blue
Coumarin
DAPI
DyLight 350
Hoechst 33258
LysoTracker Blue/MeOH

JNO-U(B) Spec

Typical Application(s) : Widefield Microscopy
Coating : Sputter/Hard Coated
Round excitation and emission filters mounted in anodized aluminum rings 2.3mm thick, up to 25mm in diameter (for standard sizes)
Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes (for standard sizes)

*** For filters larger than 25mm and dichroics larger than *** 26x38mm, please contact us for pricing.

Fluorescence, MICROSCOPY

Fluorescence microscopy

2016년 12월 15일 jhjin

형광 현미경 관찰법 (검경법)

Fluorescence Microscopy

물체에 강한 빛을 조사할 때 발현되는 형광을 이용하여 물체의 구조를 관찰하거나 형광의 유무와 색조를 이용하여 물질을 판별한는 현미경입니다.
발현되는 형광의 양을 측광하여 물질의 특성을 파악하고자 하는 경우에도 사용되고 있습니다.
일반 생물 현미경은 주로 투과 조명을 사용합니다만, 근래의 형광현미경은 주로 반사조명을 사용하고 있습니다.

형광 현미경 샘플 이미지 ( by J.H.JIN )

Fluorochrome
( Fluorophores or Fluorescent Probes)

형광은 특정 파장의 빛을 흡수하는 동시에 다른 파장(흡수 파장보다 긴 파장), 작은 에너지의 빛을 반사하는 분자현상입니다. 이 과정은 excitation(여기) 와 emission(방출, 발광) 으로 알려져 있습니다.

유기 및 비유기의 많은 물질들은 형광 특성을 가지고 있습니다. 형광 현미경을 사용하는 초기의 현미경 학자들은 1차 형광 또는 자가 형광을 주로 보았으나, 지금은 매우 밝은 형광을 가지는 많은 염료(fluorochrome)가 개발되었고 이는 시편의 특정 부분을 염색하는데 사용이 됩니다. 이러한 방법은 2차 혹은 간접 형광이라고 합니다.

이러한 염료는 형광색소(Fluorochrome) 라고 하는데, 항체와 핵산과 같은 다른 유기 합성 물질을 결합할 때는 fluorescent probes 혹은 fluorophores로 불려집니다. (하지만, 일반적으로 이러한 용어들은 종종 같은 의미로 사용되어 지고 있습니다. )

형광 현미경의 구조 및 형광의 특성

형광 현미경 ( 좌 ) 과 명시야 현미경 ( 우 ) 의 광로 비교

형광을 발현하기 위해서는 빛이 흡수가 필요합니다.
형광은 여기광의 입사방향과 관계없이 모든 방향으로 발현합니다.
일반적으로 형광의 강도는 여기광에 비교하여 매우 약합니다.
형광 파장은 여기광과 관계없이 일정합니다.
형광의 파장은 여기광(흡수광)의 파장보다 깁니다.(Stroke’s law)
형광은 정도의 차는 있지만 소광 또는 퇴색합니다.

응용분야 로는

의학,치학,약학 생물학의 기초연구 등
임상위생검사, 가축위생, 식물내해 등의 시험연구 등
화학, 약품, 반도체관련 등의 공업분야에서도 넓게 사용되어지고 있다.

표준 형광 필터

형광현미경용 광원장치

기본형광색소

Camera, Fluorescence, PHYSIOLOGY

AcquCAM 23S

2016년 4월 5일 jhjin

AcquCAM 23S

(Down load Catalog ACQUCAM 23S)

USB 3.0 Monochrome Camera
1/1.2 ” Exmor Sensor
1920×1200 pixel
Trigger input and I/O

EXMOR Sensors: Next-generation imaging sensors based on CMOS, increase the strength and reduce noise.

High sensitivity camera available from visible to IR range

20151120_1(CA1 pyramidal neuron) — Apply high sensitivity EXMOR sensor with additional filter block visible range, IR Cut filter image could be observed.

Available wider range of observation

AcquCAM 23S_6 — By applying a 1 / 1.2 ” EXMOR sensor, you can observe a wider range of superior sensitivity image.
These images were shot by AcquCAM 23S Package.

High sensitivity and fast frame image

AcquCAM 23S_(Dentate gyrus Granule cell) — To maximize the range of imaging possible observation with fast frame 54Fps and high sensitivity 0.015 lx .

[Specification]

Spec	Description
Size	1/1.2 inch
Resolution	H: 1920, V: 1200
Pixel size	H: 5.86 µm, V: 5.86 µm
Video formats & Frame rate	1920×1200 Y16 @ 25 fps 1920×1200 Y800 @ 54 fps
Sensitivity	0.015 lx
Exposure time	1/100000 to 30 s
Lens mount	C/Cs mount
Interface	USB 3.0
Power supply	4.5 to 5.5 VDC
Dimensions	H: 29 mm, W: 29 mm, L: 43 mm
Mass	65 g

AcquCAM, Camera, Fluorescence, PHYSIOLOGY

AcquCAM Pro/S3

2016년 4월 5일 jhjin

AcquCAM Pro/S3

(Down load Catalog_ACQUCAM PRO/S3)

AcquCAM Pro/S3 USB 3.0 camera is the perfect solution for many bio, industrial, medical applications. Utilizing the very sensitive mono CCD sensors, these cameras feature a specialization of fluorescence observations. With up to 30 fps and a trigger delay of less than 5 micro seconds.

1.2 Mega pixel CCDcamera
Monochrome, Progressive scan
Preview software provided (JNOPTIC Capture)
Software compatible with Windows XP/ Vista/ 7/ 8 operating systems

Apply Pseudo color for live fluorescence

Apply Pseudo color for black live fluorescence

Emphasis function for variety images

Getting clear image with general light source

[Specification]

Spec	Description
Size	1/3 inch
Resolution	H: 1280, V: 960
Pixel size	H: 3.75 µm, V: 3.75 µm
Video formats & Frame rate	1280×960 Y800 @ 30, 20, 15, 7.5, 3.75 fps 1280×960 Y16 @ 30, 20, 15, 7.5, 3.75 fps
Sensitivity	0.015 lx
Exposure time	1/10000 to 30 s
Lens mount	C/Cs mount
Interface	USB 3.0
Power supply	4.5 to 5.5 VDC
Dimensions	H: 29 mm, W: 29 mm, L: 43 mm
Mass	65 g

JNOPTIC Filter JNO-G(L)

JNO-G(L) Spectrum

주요 형광 색소(Fluorochromes)와 스펙트럼 비교

JNO-G(L)필터를 이용한 Sample Image 촬영

JNO-G(L)필터에 적합한 형광색소

JNO-G(L) Spec

JNOPTIC Filter JNO-B(L)

JNO-B(L) Spectrum

주요 형광 색소(Fluorochromes)와 스펙트럼 비교

JNO-B(L)필터를 이용한 Sample Image 촬영

JNO-B(L)필터에 적합한 형광색소

JNO-B(L) Spec

JNOPTIC Filter JNO-R2(B)

JNO-R2(B) Spectrum

주요 형광 색소(Fluorochromes)와 스펙트럼 비교

JNO-R2(B)필터에 적합한 형광색소

JNO-R2(B) Spec

JNOPTIC Filter JNO-R1(B)

JNO-R1(B) Spectrum

주요 형광 색소(Fluorochromes)와 스펙트럼 비교

JNO-R1(B)필터에 적합한 형광색소

JNO-R1(B) Spec

JNOPTIC Filter JNO-G(B)

JNO-G(B) Spectrum

주요 형광 색소(Fluorochromes)와 스펙트럼 비교

JNO-G(B)필터를 이용한 Sample Image 촬영

JNO-G(B)필터에 적합한 형광색소

JNO-G(B) Spec

JNOPTIC Filter JNO-B(B)

JNO-B(B) Spectrum

주요 형광 색소(Fluorochromes)와 스펙트럼 비교

JNO-B(B)필터를 이용한 Sample Image 촬영

JNO-B(B)필터에 적합한 형광색소

JNO-B(B) Spec

JNO-U(B) Spectrum

주요 형광 색소(Fluorochromes)와 스펙트럼 비교

* DAPI VS JNO-U(B)

* Hoechst VS JNO-U(B)

* AlexaFluor350 VS JNO-U(B)

JNO-U(B)필터를 이용한 Sample Image 촬영

JNO-U(B) 필터에 적합한 형광색소

JNO-U(B) Spec

형광 현미경 관찰법 (검경법)

Fluorescence Microscopy

Fluorochrome ( Fluorophores or Fluorescent Probes)

형광 현미경의 구조 및 형광의 특성

응용분야 로는

현미경으로 보는 광학 세상

Fluorochrome
( Fluorophores or Fluorescent Probes)