카테고리 보관물: CHROMA
for Chroma Filter
Fluorochrome – Cy7™
Cy7™
Spectrum Chart
| Excitation (㎚) | Emission (㎚) |
| 743 | 767 |
Recommended Filter set
- 일반 형광 Lighting source
| Model No. | Excitation | Dichroic mirror | Emission | Remarks |
|---|---|---|---|---|
| JNO-49007 | ET710/75x | T760lpxr | ET810/90m | – |
| JNO-49037 | ET740/40x | T770lpxr | ET780lp | – |
- Cy7을 관찰하기 위한 형광 광원 장치
- T. 02-3473-4188로 연락 주시기 바랍니다.
Information
시아닌은 여전히 업계에서, 그리고 최근에는 생명 공학 (라벨링, 분석)에서 사용되고 있습니다. 시아닌은 형광 염료로서, 특히 생체 의학 이미징에서 많은 용도로 사용되며 구조에 따라 IR에서 UV까지 스펙트럼을 커버합니다.
Cyanine은 polymethine 그룹에 속하는 합성 염료 계열의 비 계통적 이름입니다. cyanin이라는 단어는 일반적으로 청록색 ( “아쿠아”에 가까운)의 그늘을 의미하는 영어 단어 “cyan”에서 유래되었으며 다소 다른 색상 “진한 파란색 “을 의미하는 그리스어 κυάνεος / κυανοῦς kyaneos / kyanous에서 파생되었습니다.
Cy7은 육안으로는 보이지 않는 근적외선 형광체이며, Cy7.5 염료와 함께 생체 내 이미징 어플리케이션에 사용됩니다.
Cyanines (Cy)는 Fluorescein (FITC) 및 rhodamines (TRITC, RRX)와 같은 기존의 염료를 대체하여 보다 밝고 안정한 형광을 생성합니다.
Cyanines were and are still used in industry, and more recently in biotechnology (labeling, analysis). Cyanines have many uses as fluorescent dyes, particularly in biomedical imaging. Depending on the structure, they cover the spectrum from IR to UV.
Cyanine is the non-systematic name of a synthetic dye family belonging to polymethine group. The word cyanin is from the English word “cyan”, which conventionally means a shade of blue-green (close to “aqua”) and is derived from the Greek κυάνεος/κυανοῦς kyaneos/kyanous which means a somewhat different color: “dark blue”.
Cy7 is a near-IR fluor that is invisible to the naked eye (Excitation/emission maximum 750/776 ㎚). It is used in in vivo imaging applications, as well as the Cy7.5 dye.
Cyanines(Cy) replace advantageously conventional dyes such as Fluorescein(FITC) and rhodamines (TRITC, RRX), yielding brighter and more stable fluorescence.
Fluorophores
Browse By Fluorochrome
Compare filter sets by selecting the recommended/alternative set listed for each fluorochrome below.
- If you need more information, please click fluorochrome. You can see each fluorochrome information and recommended filter sets.
- If you need other dye information, please contact us.
- Tel : 02-3473-4188~9
- E-mail : jhjin@jnoptic.com
하단의 Fluorochrome 이름을 클릭하시면
대응되는 형광필터의 조합을 확인 하실 수 있습니다.
Alexa Fluor 594 dye is a bright, red-fluorescent dye that can be excited using the 561 nm or 594 nm laser lines. For stable signal generation in imaging and flow cytometry, Alexa Fluor 594 dye is pH-insensitive over a wide molar range.
Cyan Fluorescent Protein (CFP) is a versatile biological marker for monitoring physiological processes, visualizing protein localization, and detecting transgenic expression in vivo. CFP can be excited by the 405 nm laser line and is optimally detected at 485 nm.
Cy5 dye is a bright, far-red-fluorescent dye that can be excited using the 633 or 647 nm laser lines, and visualized Cy5 filter sets. In addition to immunocytochemistry applications, Cy5 is also commonly used to label nucleic acids.
A variety of cyanine 5.5 (Cy5.5) dyes has been used to label biological molecules for fluorescence imaging and other fluorescence-based biochemical analysis. They are widely used for labeling peptides, proteins and oligos etc. Cy5.5 dyes are one type of the most common red fluorophores.
Cy7 is a near-IR fluor that is invisible to the naked eye (Excitation/emission maximum 750/776 nm). It is used in in vivo imaging applications, as well as the Cy7.5 dye.
DAPI (4′,6-diamidino-2-phenylindole) is a fluorescent stain that binds strongly to A-T rich regions in DNA. It is used extensively in fluorescence microscopy. As DAPI can pass through an intact cell membrane, it can be used to stain both live and fixed cells, though it passes through the membrane less efficiently in live cells and therefore the effectiveness of the stain is lower.
Fluorescein isothiocyanate (FITC) is a derivative of fluorescein used in wide-ranging applications including flow cytometry. FITC is the original fluoresce in molecule functionalized with an isothiocyanate reactive group (-N=C=S), replacing a hydrogen atom on the bottom ring of the structure. This derivative is reactive towards nucleophiles including amine and sulfhydryl groups on proteins.
The green fluorescent protein (GFP) is a protein composed of 238 amino acid residues (26.9 kDa) that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. Although many other marine organisms have similar green fluorescent proteins, GFP traditionally refers to the protein first isolated from the jellyfish Aequorea victoria. The GFP from A. victoria has a major excitation peak at a wavelength of 395 nm and a minor one at 475 nm. Its emission peak is at 509 nm, which is in the lower green portion of the visible spectrum.
tdTomato is a genetic fusion of two copies of the dTomato gene (2) which was specifi cally designed for low aggregation (1). Its tandem dimer structure plays an important role in the exceptional brightness of tdTomato (Table I). Its excitation and emission maxima occur at 554 nm and 581 nm, respectively (1). Because tdTomato forms an intramolecular dimer, it behaves like a monomer, and has been used successfully for N- and C- terminal fusions. It shows excellent photostability and its half-time (t0.5) for maturation is one hour at 37°C.
Tetramethylrhodamine (TRITC) is a bright orange-fluorescent dye with excitation ideally suited to the 532 nm laser line. It is commonly conjugated to antibodies and proteins for cellular imaging applications.
JNO-G(L)
JNOPTIC Filter JNO-G(L)
– Filter set for TRITC / Cy3 Longpass
– Chroma Filter Model No. : 19004
JNO-G(L) Spectrum
| Filters | Type |
| AT540/25x | EX |
| AT565DC | BS |
| AT575lp | EM |
주요 형광 색소(Fluorochromes)와 스펙트럼 비교
* TRITC VS JNO-G(L)
JNO-G(L)필터를 이용한 Sample Image 촬영
JNO-G(L)필터에 적합한 형광색소
- R-phycoerythrin
JNO-G(L) Spec
- Typical Application(s) : Widefield Microscopy
- Coating : Sputter/Hard Coated
- Round excitation and emission filters mounted in anodized aluminum rings 2.3mm thick, up to 25mm in diameter (for standard sizes)
- Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes (for standard sizes)
*** For filters larger than 25mm and dichroics larger than 26x38mm, please contact us for pricing.
JNO-B(L)
JNOPTIC Filter JNO-B(L)
– Filter set for GFP / FITC Longpass
– Chroma Filter Model No. : 19002
JNO-B(L) Spectrum
| Filters | Type |
| AT480/30x | EX |
| AT505DC | BS |
| AT515lp | EM |
주요 형광 색소(Fluorochromes)와 스펙트럼 비교
* FITC VS JNO-B(L)
* GFP VS JNO-B(L)
JNO-B(L)필터를 이용한 Sample Image 촬영
JNO-B(L)필터에 적합한 형광색소
- Acridine Orange + DNA and RNA
- Auramine-rhodamine
- JC-1 non-ratiometric
JNO-B(L) Spec
- Typical Application(s) : Widefield Microscopy
- Coating : Sputter/Hard Coated
- Round excitation and emission filters mounted in anodized aluminum rings 2.3mm thick, up to 25mm in diameter (for standard sizes)
- Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes (for standard sizes)
*** For filters larger than 25mm and dichroics larger than 26x38mm, please contact us for pricing. 02-3473-4188~9
JNO-U(L)
JNOPTIC Filter JNO-U(L)
– Filter set for UV/DAPI Longpass
– Chroma Filter Model No. : 19000
JNO-U(L) Spectrum
| Filters | Type |
| AT375/28x | EX |
| AT415DC | BS |
| AT435lp | EM |
주요 형광 색소(Fluorochromes)와 스펙트럼 비교
* DAPI VS JNO-U(L)
JNO-U(L)필터를 이용한 Sample Image 촬영
JNO-U(L)에 적합한 형광색소
- Calcofluor White
- DAPI
JNO-U(L) Spec
- Typical Application(s) : Widefield Microscopy
- Coating : Sputter/Hard Coated
- Round excitation and emission filters mounted in anodized aluminum rings 2.3mm thick, up to 25mm in diameter (for standard sizes)
- Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes (for standard sizes)
*** For filters larger than 25mm and dichroics larger than 26x38mm, please contact us for pricing.
JNO-R2(B)
JNOPTIC Filter JNO-R2(B)
– Filter set for Cy5 / AlexaFluor 647 / Draq5
– Chroma Filter Model No. : 39007
JNO-R2(B) Spectrum
| Filters | Type |
| AT620/50x | EX |
| AT655DC | BS |
| AT690/50m | EM |
주요 형광 색소(Fluorochromes)와 스펙트럼 비교
* Cy5 VS JNO-R2(B)
* AlexaFluor 647 VS JNO-R2(B)
* Draq5 VS JNO-R2 (B)
JNO-R2(B)필터에 적합한 형광색소
- Alexa Fluor 647™
- Atto 647N
- Cy5™
- DiD
- Draq5
- DyLight 649
- MitoTracker Deep Red 633/MeOH
- Nile Blue
- Quasar® 670
- SYTO® 60
- TO-PRO™-3
JNO-R2(B) Spec
- Typical Application(s) : Widefield Microscopy
- Coating : Sputter/Hard Coated
- Round excitation and emission filters mounted in anodized aluminum rings 2.3mm thick, up to 25mm in diameter (for standard sizes)
- Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes (for standard sizes)
*** For filters larger than 25mm and dichroics larger than 26x38mm, please contact us for pricing.
JNO-R1(B)
JNOPTIC Filter JNO-R1(B)
– Filter set for Texas Red / mCherry / AlexaFluor 594
– Chroma Filter Model No. : 39010
JNO-R1(B) Spectrum
| Filters | Type |
| AT560/40x | EX |
| AT600DC | BS |
| AT635/60m | EM |
주요 형광 색소(Fluorochromes)와 스펙트럼 비교
* AlexaFluor 594 VS JNO-R1(B)
* Texas Red VS JNO-R1(B)
* mCherry VS JNO-R1(B)
JNO-R1(B)필터에 적합한 형광색소
- Alexa Fluor 568™
- Alexa Fluor 594™
- CAL Fluor® Red 610
- Cy3.5™
- DyLight 594
- FusionRed
- Killer Red
- mCherry
- MitoTracker Red/MeOH
- mKate2
- mRFP1
- ReAsH-CCPGCC
- Rhodamine Red™-X
- ROX
- Sulforhodamine 101
- Texas Red®
- Texas Red®-X
- X-rhod-1/Ca2+
JNO-R1(B) Spec
- Typical Application(s) : Widefield Microscopy
- Coating : Sputter/Hard Coated
- Round excitation and emission filters mounted in anodized aluminum rings 2.3mm thick, up to 25mm in diameter (for standard sizes)
- Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes (for standard sizes)
*** For filters larger than 25mm and dichroics larger than 26x38mm, please contact us for pricing.
JNO-G(B)
JNOPTIC Filter JNO-G(B)
– Filter set for TRITC / Cy3 / TagRFP / AlexaFluor546
– Chroma Filter Model No. : 39004
JNO-G(B) Spectrum
| Filters | Type |
| AT540/25x | EX |
| AT565DC | BS |
| AT605/55m | EM |
주요 형광 색소(Fluorochromes)와 스펙트럼 비교
* TRITC VS JNO-G(B)
* Cy3 VS JNO-G(B)
* AlexaFluor546 VS JNO-G(B)
JNO-G(B)필터를 이용한 Sample Image 촬영
JNO-G(B)필터에 적합한 형광색소
- Alexa Fluor 546™
- Alexa Fluor 555™
- Atto 550
- CAL Fluor® Red 590
- Cy3™
- DiI
- DsRed
- DyLight 549
- MitoTracker Orange/MeOH
- Resorufin
- Rhod-2
- TagRFP
- TAMRA
- tdTomato
- Tetramethylrhodamine isothiocyanate
- TRITC
JNO-G(B) Spec
- Typical Application(s) : Widefield Microscopy
- Coating : Sputter/Hard Coated
- Round excitation and emission filters mounted in anodized aluminum rings 2.3mm thick, up to 25mm in diameter (for standard sizes)
- Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes (for standard sizes)
*** For filters larger than 25mm and dichroics larger than 26x38mm, please contact us for pricing.
JNO-B(B)
JNOPTIC Filter JNO-B(B)
– Filter set for EGFP / FITC / Cy2 / AlexaFluor488
– Chroma Filter Model No. : 39002
JNO-B(B) Spectrum
| Filters | Type |
| AT480/30x | EX |
| AT505DC | BS |
| AT535/40m | EM |
주요 형광 색소(Fluorochromes)와 스펙트럼 비교
* FITC VS JNO-B(B)
* GFP VS JNO-B(B)
* AlexaFluor488 VS JNO-B(B)
JNO-B(B)필터를 이용한 Sample Image 촬영
JNO-B(B)필터에 적합한 형광색소
- Acridine Orange + DNA
- Alexa Fluor 488™
- Atto 488
- Azami Green
- BODIPY FL/pH7.2
- Calcein
- Calcium Green™-1
- Cy2™
- DiO
- DyLight 488
- EGFP
- Emerald GFP
- FAM
- FITC
- Fluo-4
- GFP
- LysoTracker Green/pH 5.2
- MitoTracker Green FM/MeOH
- mWasabi
- Oregon Green™ 488
- SYBR® Green I
- SYTO 9/DNA
- ZsGreen1
JNO-B(B) Spec
- Typical Application(s) : Widefield Microscopy
- Coating : Sputter/Hard Coated
- Round excitation and emission filters mounted in anodized aluminum rings 2.3mm thick, up to 25mm in diameter (for standard sizes)
- Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes (for standard sizes)
*** For filters larger than 25mm and dichroics larger than 26x38mm, please contact us for pricing. ( Tel. 02-3473-4188)